Human/Mouse CCR6 PE-conjugated Antibody

(13 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5. Detects human CCR6 in flow cytometry.
  • Source
    Monoclonal Rat IgG2A Clone # 140706
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
    Met1-Met367
    Accession # O54689
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Human/Mouse CCR6 PE‑conjugated Monoclonal Antibody (Catalog # FAB590P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR6 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Rat Anti-Human/Mouse CCR6 PE‑conjugated Monoclonal Antibody (Catalog # FAB590P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

  • Entrez Gene IDs:
    1235 (Human); 12458 (Mouse); 308163 (Rat)
  • Alternate Names:
    BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The chemokine receptor CCR6 facilitates the onset of mammary neoplasia in the MMTV-PyMT mouse model via recruitment of tumor-promoting macrophages.
    Authors: Boyle S, Faulkner J, McColl S, Kochetkova M
    Mol Cancer, 2015;14(0):115.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Flow
  2. Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFkappaB and ERK.
    Authors: Badr G, Sayed A, Abdel-Maksoud M, Mohamed A, El-Amir A, Abdel-Ghaffar F, Al-Quraishy S, Mahmoud M
    PLoS ONE, 2015;10(4):e0125340.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Differential developmental requirement and peripheral regulation for dermal Vgamma4 and Vgamma6T17 cells in health and inflammation.
    Authors: Cai Y, Xue F, Fleming C, Yang J, Ding C, Ma Y, Liu M, Zhang H, Zheng J, Xiong N, Yan J
    Nat Commun, 2014;5(0):3986.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Cryptococcus gattii infection dampens Th1 and Th17 responses by attenuating dendritic cell function and pulmonary chemokine expression in the immunocompetent hosts.
    Authors: Angkasekwinai P, Sringkarin N, Supasorn O, Fungkrajai M, Wang Y, Chayakulkeeree M, Ngamskulrungroj P, Angkasekwinai N, Pattanapanyasat K
    Infect Immun, 2014;82(9):3880-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis.
    Authors: Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, Hacker G, Schmetzer H, Hacker H, Hammerschmidt S, van der Ende A, Pfister H, van de Beek D, Koedel U
    PLoS ONE, 2014;9(4):e93057.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Liver-resident NK cells confer adaptive immunity in skin-contact inflammation.
    Authors: Peng, Hui, Jiang, Xiaojun, Chen, Yonglin, Sojka, Dorothy, Wei, Haiming, Gao, Xiang, Sun, Rui, Yokoyama, Wayne M, Tian, Zhigang
    J Clin Invest, 2013;123(4):1444-56.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Difference in Th1 and Th17 lymphocyte adhesion to endothelium.
    Authors: Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH
    J. Immunol., 2012;188(3):1421-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  8. Notch, Id2, and ROR gamma-t sequentially orchestrate the fetal development of lymphoid tissue inducer cells.
    Authors: Cherrier M, Sawa S, Eberl G
    J. Exp. Med., 2012;209(4):729-40.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
    Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN
    J. Immunol., 2009;182(11):6851-60.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  10. ATP drives lamina propria T(H)17 cell differentiation.
    Authors: Atarashi K, Nishimura J, Shima T, Umesaki Y, Yamamoto M, Onoue M, Yagita H, Ishii N, Evans R, Honda K, Takeda K
    Nature, 2008;455(7214):808-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  11. Dendritic cells in islets of Langerhans constitutively present beta cell-derived peptides bound to their class II MHC molecules.
    Authors: Calderon B, Suri A, Miller MJ
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(16):6121-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  12. Both Th1 and Th17 are immunopathogenic but differ in other key biological activities.
    Authors: Cox CA, Shi G, Yin H, Vistica BP, Wawrousek EF, Chan CC, Gery I
    J. Immunol., 2008;180(11):7414-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  13. Chemokine receptor CCR2 but not CCR5 or CCR6 mediates the increase in pulmonary dendritic cells during allergic airway inflammation.
    Authors: Robays LJ, Maes T, Lebecque S, Lira SA, Kuziel WA, Brusselle GG, Joos GF, Vermaelen KV
    J. Immunol., 2007;178(8):5305-11.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 13 Citations
Isotype Controls
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Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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