Mouse CCR6 Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB590T-100UG
Detection of CCR6 in Mouse Th17-Differentiated Splenocytes by Flow Cytometry.
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Mouse CCR6 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5.
Source
Monoclonal Rat IgG2A Clone # 140706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
Met1-Met367
Accession # O54689
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR6 in Mouse Th17-Differentiated Splenocytes by Flow Cytometry. View Larger

Detection of CCR6 in Mouse Th17-Differentiated Splenocytes by Flow Cytometry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3 epsilon  monoclonal antibody (Catalog # MAB484, 5 μg/mL) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 μg/mL), Recombinant Human TGF‑ beta 1 (Catalog # 240-B, 10 ng/mL) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/mL), Recombinant Mouse IL‑6 (Catalog # 406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 μg/mL) for 5 days were stained with (A) Rat Anti-Mouse CCR6 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB590T) or (B) RatIgG2A isotype control antibody (Catalog # IC006T) and Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Entrez Gene IDs
1235 (Human); 12458 (Mouse); 308163 (Rat)
Alternate Names
BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22

Product Datasheets

FAQs

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