Mouse CCR6 Alexa Fluor® 594-conjugated Antibody

FAB590T Datasheet / COA / SDS

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FAB590T-100UG

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Detection of CCR6 in Mouse Splenocytes by Flow Cytometry.

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Mouse CCR6 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5.

Source

Monoclonal Rat IgG_2A Clone # 140706

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
Met1-Met367
Accession # O54689

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry

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Detection of CCR6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse CCR6 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB590T) or (B) RatIgG2A isotype control antibody (IC006T) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (FAB1217A). Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry

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Detection of CCR6 in Mouse Th17-Differentiated Splenocytes by Flow Cytometry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3 epsilon monoclonal antibody (Catalog # MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 µg/mL), Recombinant Human TGF- beta 1 (Catalog # 240-B, 10 ng/mL) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (Catalog # 406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 µg/mL) for 5 days were stained with (A) Rat Anti-Mouse CCR6 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB590T) or (B) RatIgG2A isotype control antibody (Catalog # IC006T) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (FAB554A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Entrez Gene IDs

1235 (Human); 12458 (Mouse); 308163 (Rat)

Alternate Names

BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKRL3; CKR-L3; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPRCY4; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22