Mouse CCR6 Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry View Larger

Detection of CCR6 in Mouse Th17-Differentiated Splenocytes by Flow Cytometry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3 epsilon  monoclonal antibody (Catalog # MAB484, 5 μg/mL) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 μg/mL), Recombinant Human TGF‑ beta 1 (Catalog # 240-B, 10 ng/mL) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/mL), Recombinant Mouse IL‑6 (Catalog # 406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 μg/mL) for 5 days were stained with (A) Rat Anti-Mouse CCR6 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB590T) or (B) RatIgG2A isotype control antibody (Catalog # IC006T) and Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Product Datasheets

Related Research Areas

• B Cell Chemotaxis, Migration, and Adhesion
• CC Chemokines and Receptors
• Chemokine Receptors
• Chemokines and Receptors in Allergy and Asthma
• Chemokines and Receptors in Angiogenesis
• Dendritic Cell Adhesion and Migration
• gamma delta T Cells
• LTi Cells
• Monocyte Markers
• Natural Killer T (NKT) Cells
• Regulatory T Cells (Tregs)
• T Cell Migration/Adhesion
• Th17 Cells
• Th22 Cells