Mouse CCR6 Alexa Fluor® 594-conjugated Antibody Summary
Accession # O54689
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CCR6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse CCR6 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB590T) or (B) RatIgG2A isotype control antibody (IC006T) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (FAB1217A). Staining was performed using our Staining Membrane-associated Proteins protocol.
Detection of CCR6 in Mouse Th17-Differentiated Splenocytes by Flow Cytometry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3 epsilon monoclonal antibody (Catalog # MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 µg/mL), Recombinant Human TGF- beta 1 (Catalog # 240-B, 10 ng/mL) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (Catalog # 406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 µg/mL) for 5 days were stained with (A) Rat Anti-Mouse CCR6 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB590T) or (B) RatIgG2A isotype control antibody (Catalog # IC006T) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (FAB554A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.
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