Human/Mouse CCR6 Alexa Fluor® 488-conjugated Antibody

(1 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5. Detects human CCR6 in flow cytometry.
  • Source
    Monoclonal Rat IgG2A Clone # 140706
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
    Met1-Met367
    Accession # O54689
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) untreated or (B) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Mouse IL‑23 (Catalog # 1887-ML) overnight to induce Th17 activation were stained with Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse CCR6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB590G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR6 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Rat Anti-Human/Mouse CCR6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB590G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

  • Entrez Gene IDs:
    1235 (Human); 12458 (Mouse); 308163 (Rat)
  • Alternate Names:
    BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.
    Authors: Funakoshi, Shuichi, Shimizu, Takeyuki, Numata, Osamu, Ato, Manabu, Melchers, Fritz, Ohnishi, Kazuo
    PLoS ONE, 2015;10(1):e0117566.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

Ctrl IC006G  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human/Mouse CCR6 Alexa Fluor® 488-conjugated Antibody FAB590G
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 Flow Mouse Anonymous 08/18/2017
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Flow Cytometry Human/Mouse CCR6 Alexa Fluor® 488-conjugated Antibody FAB590G
Flow Cytometry: Human/Mouse CCR6 Alexa Fluor® 488-conjugated Antibody [FAB590G]

Summary

ApplicationFlow Cytometry
Sample TestedMouse splenocytes
SpeciesMouse

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