Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 140706
View all CCR6 Primary Antibodies »

Product Specifications

Immunogen

RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
Met1-Met367
Accession # O54689

Specificity

Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5. Detects human CCR6 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse CCR6 Alexa Fluor® 488‑conjugated Antibody

Detection of CCR6 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CCR6 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) and either (A) Rat Anti-Human/Mouse CCR6 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB590G) or (B) Rat IgG2AAlexa Fluor 488 Isotype Control (IC006G). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR6 in B6 splenocytes by Flow Cytometry.

B6 splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Human/Mouse CCR6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB590G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse CCR6 Alexa Fluor® 488‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Samples used for this experiment were Human peripheral blood lymphocytes and B6 splenocytes 

Reviewed Applications

Read 1 review rated 5 using FAB590G in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Alternate Names

CC-CKR-6, CCR6, CD196, CKR-L3, CKRL3, CMKBR6, DRY6, GPR-CY4, GPR29, GPRCY4, STRL22

Entrez Gene IDs

1235 (Human); 12458 (Mouse); 308163 (Rat)

Gene Symbol

CCR6

UniProt

O54689

Additional CCR6 Products

Product Documents for Human/Mouse CCR6 Alexa Fluor® 488‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse CCR6 Alexa Fluor® 488‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Citations for Human/Mouse CCR6 Alexa Fluor® 488‑conjugated Antibody

Customer Reviews for Human/Mouse CCR6 Alexa Fluor® 488‑conjugated Antibody (1)

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  • Human/Mouse CCR6 Alexa Fluor® 488-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Mouse splenocytes
    Species: Mouse
    Verified Customer | Posted 08/18/2017
    Human/Mouse CCR6 Alexa Fluor® 488‑conjugated Antibody FAB590G

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