Mouse Gr-1/Ly-6G Fluorescein-conjugated Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Gr-1/Ly-6G. Weak cross-reactivity with Ly-6C is observed.
  • Source
    Monoclonal Rat IgG2B Clone # RB6-8C5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Gr‑1/Ly‑6G in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse Gr‑1/Ly‑6G Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1037F) or (B) Rat IgG2B Fluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Ly-6G/Ly-6C (Gr-1)
The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).
  • References:
    1. Spangrude, G.J. et al. (1988) Science 241:58.
    2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
    3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
    4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
  • Long Name:
    A Myeloid Differentiation Antigen
  • Entrez Gene IDs:
    546644 (Mouse); 546644 (Mouse)
  • Alternate Names:
    Ly-6C; Ly6G; Ly-6G; Ly-6G/Ly-6C (Gr-1)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Species
Applications
Sample Type
  1. MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1.
    Authors: Salvucci O, Jiang K, Gasperini P
    Haematologica, 2012;97(6):818-26.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. IL-17RA signaling amplifies antibody-induced arthritis.
    Authors: Sadik CD, Kim ND, Alekseeva E, Luster AD
    PLoS ONE, 2011;6(10):e26342.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Chemokine homeostasis vs. chemokine presentation during severe acute lung injury: the other side of the Duffy antigen receptor for chemokines.
    Authors: Zarbock A, Bishop J, Muller H, Schmolke M, Buschmann K, Van Aken H, Singbartl K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(3):L462-71.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. TREM-1 promotes survival during septic shock in mice.
    Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
    Eur. J. Immunol., 2007;37(2):456-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Fluorescein-conjugated Antibody

Ctrl IC013F 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse Gr-1/Ly-6G Fluorescein-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information