Detection of Gr‑1/Ly‑6G in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse Gr‑1/Ly‑6G Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1037F) or (B) Rat IgG2B Fluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Ly-6G/Ly-6C (Gr-1)
The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The
RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells
(2). In the periphery, this antibody specifically recognized granulocytes (3, 4).
Spangrude, G.J. et al. (1988) Science 241:58.
Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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