Mouse CD300a/LMIR1 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse CD300a/LMIR1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LMIR1, 2, 3, 4, 5, 6, recombinant mouse LMIR3, 4, or 5 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 172224
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD300a/LMIR1
    Accession # BAC80268
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD300a/LMIR1 in T1165 Mouse Cell Line by Flow Cytometry. T1165 mouse plasmacytoma cell line was stained with Rat Anti-Mouse CD300a/LMIR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB1186P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD300a/LMIR1
CD300a, also known as LMIR1, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse CD300a consists of a 158 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 112 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2, 3). Within the ECD, mouse CD300a shares 40% and 66% aa sequence identity with human and rat CD300a, respectively. Alternate splicing generates an additional mouse CD300a isoform with a 4 aa deletion following the Ig-like domain (3). In mouse, CD300a is expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells (2‑4). Antibody cross‑linking of CD300a induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Cross‑linking of CD300a to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (4, 8‑10). CD300a cross‑linking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 7, 9).
  • References:
    1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
    2. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
    3. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
    4. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.
    5. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
    6. Munitz, A. et al. (2006) Blood 107:1996.
    7. Bachelet, I. et al. (2005) J. Immunol. 175:7989.
    8. Bachelet, I. et al. (2008) J. Immunol. 180:6064.
    9. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
    10. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.
  • Entrez Gene IDs:
    11314 (Human); 217303 (Mouse)
  • Alternate Names:
    CD300a antigenCMRF35H9; CD300a molecule; CD300a; CLM8; CLM-8; CMRF35H leukocyte immunoglobulin-like receptor; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF-35-H9CD300 antigen-like family member A; CMRF35HIRp60; CMRF35-like molecule 8; IgSF12; IGSF12NK inhibitory receptor; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 13  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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