Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, Engagement Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 172224
View all CD300a/LMIR1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD300a/LMIR1
Leu28-Arg183
Accession # BAC80268

Specificity

Detects mouse CD300a/LMIR1 in direct ELISAs. In direct ELISAs, approximately 2% cross-reactivity with recombinant mouse (rm) LMIR2 is observed and no cross-reactivity with recombinant human LMIR1, 2, 3, 4, 5, 6, or rmLMIR3, 4, or 5 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse CD300a/LMIR1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse T1165 plasmocytoma cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD300a/LMIR1

CD300a, also known as LMIR1, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse CD300a consists of a 158 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 112 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2, 3). Within the ECD, mouse CD300a shares 40% and 66% aa sequence identity with human and rat CD300a, respectively. Alternate splicing generates an additional mouse CD300a isoform with a 4 aa deletion following the Ig-like domain (3). In mouse, CD300a is expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells (2‑4). Antibody cross‑linking of CD300a induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Cross‑linking of CD300a to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (4, 8‑10). CD300a cross‑linking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 7, 9).

References

  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
  3. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
  4. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.
  5. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
  6. Munitz, A. et al. (2006) Blood 107:1996.
  7. Bachelet, I. et al. (2005) J. Immunol. 175:7989.
  8. Bachelet, I. et al. (2008) J. Immunol. 180:6064.
  9. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
  10. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.

Alternate Names

CD300a, CLM8, CMRF-35H, IGSF12, IRC1, IRC2, IRp60, LMIR1, MAIR-I, Mcipr1, NKRL, Pigr4

Entrez Gene IDs

11314 (Human); 217303 (Mouse)

Gene Symbol

CD300A

UniProt

BAC80268

Additional CD300a/LMIR1 Products

Product Documents for Mouse CD300a/LMIR1 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CD300a/LMIR1 Antibody

For research use only

Citations for Mouse CD300a/LMIR1 Antibody

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