Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all CD300g/Nepmucin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD300g/Nepmucin
Leu19-Ile284
Accession # NP_001154183

Specificity

Detects mouse CD300g/Nepmucin in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CD300g/Nepmucin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse CD300g/Nepmucin Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD300g/Nepmucin

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD300g/Nepmucin

Nepmucin (a not expressed in Peyer's Patch mucin; also CLM-9, CD300g and TREM4) is an 80‑95 kDa member of the CD300L family of proteins. It is expressed on endothelium and serves multiple functions. It is known to form homotypic interactions between endothelial cells, bind lymphocytes via its Ig-like and mucin domains and serve as a transcellular transport vehicle for IgA and IgM. Mature mouse Nepmucin is a 397 amino acid (aa) type I transmembrane glycoprotein. Its extracellular region (aa 19‑288) contains one V-type Ig-like domain (aa 19‑124) plus an O-glycosylated mucin region (aa 136‑276). There are three isoform variants. One is 60‑75 kDa in size and shows an Ala substitution for aa 126‑170, a second is 46‑50 kDa in size and shows an Arg substitution for aa 170‑254, while a third is 36 kDa in size and shows a SerTyr substitution for aa 134‑256. Over aa 1‑284, mouse Nepmucin shares 89% and 53% aa identity with rat and human Nepmucin, respectively.

Alternate Names

CD300LG, CLM9, Nepmucin, TREM-4

Entrez Gene IDs

146894 (Human); 52685 (Mouse); 684984 (Rat)

Gene Symbol

CD300LG

UniProt

NP_001154183

Additional CD300g/Nepmucin Products

Product Documents for Mouse CD300g/Nepmucin Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CD300g/Nepmucin Biotinylated Antibody

For research use only

Related Research Areas

Endothelial Cell Markers

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