Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Neutralization, Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 829706
View all CD74 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD74
Gln56-Cys215
Accession # P04441

Specificity

Detects mouse CD74 in ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse CD74 APC‑conjugated Antibody

Detection of CD74 antibody in A20 Mouse Cell Line antibody by Flow Cytometry.

Detection of CD74 in A20 Mouse Cell Line by Flow Cytometry.

A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse CD74 APC-conjugated Monoclonal Antibody (Catalog # FAB7478A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD74 by Flow Cytometry

Detection of CD74 by Flow Cytometry

Expansion of HSPCs in the BM of CD74−/− mice.(A) BM cells derived from WT or CD74−/− were purified. Histograms show representative analysis of CD74 expression on HSPCs in WT and CD74−/− mice. n = 3. (B) Total BM cellularity per femur and tibia in WT and CD74−/− mice, Data A in S1 Data. (C–J) The percent of the different populations in WT and CD74−/−-derived BM cells. (C) Lin-; Data B in S1 Data(D) Representative FACS analysis of WT and CD74−/− HSPCs; (E) LSK; Data C in S1 Data(F) CD34-/LSK; Data D in S1 Data and (G) CD34+; Data E in S1 Data(H) CD150+CD48-LSK; Data F in S1 Data(I) CD150-CD48-/LSK; Data G in S1 Data and (J) CD150-CD48+/LSK; n = 14–18, Data H in S1 Data. (K) CFUC assay: Total BM cells from WT and CD74−/− mice were seeded at 15,000 cells/mL in semisolid cultures supplemented with cytokines and nutrients. CFU-C were counted 7 days later; n = 7, Data I in S1 Data. Bars show SEM. Unpaired two-tailed t test *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. The fcs files and gates can be found at the Flow Repository (accession number FR-FCM-Z3F2). BM, bone marrow; CFU-C, colony-forming unit cell; FACS, fluorescence-activated cell sorting; HSPC, hematopoietic stem and progenitor cell; WT, wild-type. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33661886), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CD74 by Flow Cytometry

Detection of CD74 by Flow Cytometry

Expansion of HSPCs in the BM of CD74−/− mice.(A) BM cells derived from WT or CD74−/− were purified. Histograms show representative analysis of CD74 expression on HSPCs in WT and CD74−/− mice. n = 3. (B) Total BM cellularity per femur and tibia in WT and CD74−/− mice, Data A in S1 Data. (C–J) The percent of the different populations in WT and CD74−/−-derived BM cells. (C) Lin-; Data B in S1 Data(D) Representative FACS analysis of WT and CD74−/− HSPCs; (E) LSK; Data C in S1 Data(F) CD34-/LSK; Data D in S1 Data and (G) CD34+; Data E in S1 Data(H) CD150+CD48-LSK; Data F in S1 Data(I) CD150-CD48-/LSK; Data G in S1 Data and (J) CD150-CD48+/LSK; n = 14–18, Data H in S1 Data. (K) CFUC assay: Total BM cells from WT and CD74−/− mice were seeded at 15,000 cells/mL in semisolid cultures supplemented with cytokines and nutrients. CFU-C were counted 7 days later; n = 7, Data I in S1 Data. Bars show SEM. Unpaired two-tailed t test *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. The fcs files and gates can be found at the Flow Repository (accession number FR-FCM-Z3F2). BM, bone marrow; CFU-C, colony-forming unit cell; FACS, fluorescence-activated cell sorting; HSPC, hematopoietic stem and progenitor cell; WT, wild-type. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33661886), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CD74 by Flow Cytometry

Detection of CD74 by Flow Cytometry

CD74 can serve as a potential target for therapy.(A, B) WT and CD74−/− BM cells were cultured alone or incubated with blocking anti-CD74 antibody (20, 50, and 100 μg/ml). After 48 h, percent LSK from live cells was analyzed by FACS; n = 4–7, Data A and B in S8 Data. (C, D) Survival curve: 5-FU (150 mg/kg and 125mg/kg) was injected to WT and CD74−/− mice once a week. Log-rank test *<0.05; n = 10 in each group, Data C and D in S8 Data. (G) Human CD34+ BM cells were stained for CD74 cell surface expression by FACS; n = 5. (H) Human BM were cultured with anti-CD74 (LN2) antibody or isotype control for 48 h, and percent of CD18 on human CD34+ cells was analyzed by FACS; n = 5, Data E in S8 Data. Bars show SEM. Unpaired two-tailed t test *p < 0.05; **p < 0.01; ***p < 0.001. The fcs files and gates can be found at the Flow Repository (accession number FR-FCM-Z3F2). 5-FU, 5-fluorouracil; BM, bone marrow; FACS, fluorescence-activated cell sorting; WT, wild-type. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33661886), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CD74 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: A20 mouse B cell lymphoma cell line

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD74

CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer (1). Mature mouse CD74 consists of a 29 amino acid (aa) cytoplasmic domain, a 29 aa transmembrane segment, and a 224 aa extracellular domain (ECD) that contains one thyroglobulin type 1 domain (2). Alternate splicing generates a short isoform that lacks the thyroglobulin domain (2). Within the ECD, mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading (3). Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface (4, 5). CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up-regulation of angiogenic factors in endometrial stromal cells (6-11). MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation (10). CD74 is upregulated on non-immune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques (12, 13). It is also upregulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis (1, 14).

References

  1. Beswick, E.J. and V.E. Reyes (2009) World J. Gastroenterol. 15:2855.
  2. Koch, N. et al. (1987) EMBO J. 6:1677.
  3. Riberdy, J.M. et al. (1992) Nature 360:474.
  4. Koch, N. et al. (1991) J. Immunol. 147:2643.
  5. Roche, P.A. et al. (1991) Nature 354:392.
  6. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  7. Takahashi, K. et al. (2009) Respir. Res. 10:33.
  8. Heinrichs, D. et al. (2011) Proc. Natl. Acad. Sci. 104:17444.
  9. Shi, X. et al. (2006) Immunity 25:595.
  10. Gore, Y. et al. (2008) J. Biol. Chem. 283:2784.
  11. Veillat, V. et al. (2010) J. Clin. Endocrinol. Metab. 95:E403.
  12. Bryan, K.J. et al. (2008) Mol. Neurodegen. 3:13.
  13. Martin-Ventura, J.L. et al. (2009) Cardiovasc. Res. 83:586.
  14. Liu, Y.-H. et al. (2008) J. Immunol. 181:6584.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Entrez Gene IDs

972 (Human); 16149 (Mouse); 25599 (Rat)

Gene Symbol

CD74

UniProt

P04441

Additional CD74 Products

Product Documents for Mouse CD74 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CD74 APC‑conjugated Antibody

For research use only

Citations for Mouse CD74 APC‑conjugated Antibody

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