Mouse CD74 APC-conjugated Antibody
Mouse CD74 APC-conjugated Antibody Summary
Accession # P04441
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD74 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse CD74 APC-conjugated Monoclonal Antibody (Catalog # FAB7478A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer (1). Mature mouse CD74 consists of a 29 amino acid (aa) cytoplasmic domain, a 29 aa transmembrane segment, and a 224 aa extracellular domain (ECD) that contains one thyroglobulin type 1 domain (2). Alternate splicing generates a short isoform that lacks the thyroglobulin domain (2). Within the ECD, mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading (3). Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface (4, 5). CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up-regulation of angiogenic factors in endometrial stromal cells (6-11). MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation (10). CD74 is upregulated on non-immune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques (12, 13). It is also upregulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis (1, 14).
- Beswick, E.J. and V.E. Reyes (2009) World J. Gastroenterol. 15:2855.
- Koch, N. et al. (1987) EMBO J. 6:1677.
- Riberdy, J.M. et al. (1992) Nature 360:474.
- Koch, N. et al. (1991) J. Immunol. 147:2643.
- Roche, P.A. et al. (1991) Nature 354:392.
- Leng, L. et al. (2003) J. Exp. Med. 197:1467.
- Takahashi, K. et al. (2009) Respir. Res. 10:33.
- Heinrichs, D. et al. (2011) Proc. Natl. Acad. Sci. 104:17444.
- Shi, X. et al. (2006) Immunity 25:595.
- Gore, Y. et al. (2008) J. Biol. Chem. 283:2784.
- Veillat, V. et al. (2010) J. Clin. Endocrinol. Metab. 95:E403.
- Bryan, K.J. et al. (2008) Mol. Neurodegen. 3:13.
- Martin-Ventura, J.L. et al. (2009) Cardiovasc. Res. 83:586.
- Liu, Y.-H. et al. (2008) J. Immunol. 181:6584.
Citations for Mouse CD74 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Targeting CD74 in B-cell non-Hodgkin lymphoma with the antibody-drug conjugate STRO-001
Authors: X Li, C Abrahams, A Yu, M Embry, R Henningsen, V DeAlmeida, S Matheny, T Kline, A Yam, R Stafford, T Hallam, M Lupher, A Molina
Sample Types: Whole Cells
Applications: Flow Cytometry
MIF promotes B cell chemotaxis through the receptors CXCR4 and CD74 and ZAP-70 signaling.
Authors: Klasen C, Ohl K, Sternkopf M, Shachar I, Schmitz C, Heussen N, Hobeika E, Levit-Zerdoun E, Tenbrock K, Reth M, Bernhagen J, El Bounkari O
J Immunol, 2014-04-23;192(11):5273-84.
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Mouse CD74 APC-conjugated Antibody
There are currently no reviews for this product. Be the first to review Mouse CD74 APC-conjugated Antibody and earn rewards!
Have you used Mouse CD74 APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image