Mouse CD74 Antibody

Catalog # Availability Size / Price Qty
MAB7478
MAB7478-SP
Detection of CD74 in A20 Mouse Cell Line by Flow Cytometry.
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Mouse CD74 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD74 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD74 is observed.
Source
Monoclonal Rat IgG1 Clone # 829706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD74
Gln56-Cys215
Accession # P04441
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD74 antibody in A20 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CD74 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse CD74 Monoclonal Antibody (Catalog # MAB7478, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD74

CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer (1). Mature mouse CD74 consists of a 29 amino acid (aa) cytoplasmic domain, a 29 aa transmembrane segment, and a 224 aa extracellular domain (ECD) that contains one thyroglobulin type 1 domain (2). Alternative splicing generates a short isoform that lacks the thyroglobulin domain (2). Within the ECD, mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading (3). Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface (4, 5). CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up‑regulation of angiogenic factors in endometrial stromal cells (6‑11). MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation (10). CD74 is upregulated on non‑immune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques (12, 13). It is also upregulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis (1, 14).

References
  1. Beswick, E.J. and V.E. Reyes (2009) World J. Gastroenterol. 15:2855.
  2. Koch, N. et al. (1987) EMBO J. 6:1677.
  3. Riberdy, J.M. et al. (1992) Nature 360:474.
  4. Koch, N. et al. (1991) J. Immunol. 147:2643.
  5. Roche, P.A. et al. (1991) Nature 354:392.
  6. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  7. Takahashi, K. et al. (2009) Respir. Res. 10:33.
  8. Heinrichs, D. et al. (2011) Proc. Natl. Acad. Sci. USA 104:17444.
  9. Shi, X. et al. (2006) Immunity 25:595.
  10. Gore, Y. et al. (2008) J. Biol. Chem. 283:2784.
  11. Veillat, V. et al. (2010) J. Clin. Endocrinol. Metab. 95:E403.
  12. Bryan, K.J. et al. (2008) Mol. Neurodegen. 3:13.
  13. Martin-Ventura, J.L. et al. (2009) Cardiovasc. Res. 83:586.
  14. Liu, Y.-H. et al. (2008) J. Immunol. 181:6584.
Entrez Gene IDs
972 (Human); 16149 (Mouse); 25599 (Rat)
Alternate Names
CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated); CD74 antigen; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74; CLIP; DHLAG; DHLAGgamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-gamma; Ii; INVG34; MHC HLA-DR gamma chain; p33

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