Detection of CD74 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse CD74 Monoclonal Antibody (Catalog # MAB7478, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer (1). Mature mouse CD74 consists of a 29 amino acid (aa) cytoplasmic domain, a 29 aa transmembrane segment, and a 224 aa extracellular domain (ECD) that contains one thyroglobulin type 1 domain (2). Alternative splicing generates a short isoform that lacks the thyroglobulin domain (2). Within the ECD, mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading (3). Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface (4, 5). CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up‑regulation of angiogenic factors in endometrial stromal cells (6‑11). MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation (10). CD74 is upregulated on non‑immune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques (12, 13). It is also upregulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis (1, 14).
Beswick, E.J. and V.E. Reyes (2009) World J. Gastroenterol. 15:2855.
Koch, N. et al. (1987) EMBO J. 6:1677.
Riberdy, J.M. et al. (1992) Nature 360:474.
Koch, N. et al. (1991) J. Immunol. 147:2643.
Roche, P.A. et al. (1991) Nature 354:392.
Leng, L. et al. (2003) J. Exp. Med. 197:1467.
Takahashi, K. et al. (2009) Respir. Res. 10:33.
Heinrichs, D. et al. (2011) Proc. Natl. Acad. Sci. USA 104:17444.
Shi, X. et al. (2006) Immunity 25:595.
Gore, Y. et al. (2008) J. Biol. Chem. 283:2784.
Veillat, V. et al. (2010) J. Clin. Endocrinol. Metab. 95:E403.
Bryan, K.J. et al. (2008) Mol. Neurodegen. 3:13.
Martin-Ventura, J.L. et al. (2009) Cardiovasc. Res. 83:586.
Liu, Y.-H. et al. (2008) J. Immunol. 181:6584.
Entrez Gene IDs:
972 (Human); 16149 (Mouse); 25599 (Rat)
CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated); CD74 antigen; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74; CLIP; DHLAG; DHLAGgamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-gamma; Ii; INVG34; MHC HLA-DR gamma chain; p33
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.