Detection of CD9 in D3 Mouse Cell Line by Flow Cytometry.
D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CD9 APC‑conjugated Monoclonal Antibody (Catalog # FAB5218A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD9 is a 22-25 kDa member of the tetraspanin (TM4SF) superfamily of molecules. Although typology of this family shows a cytoplasmic N- and C-terminus, tetraspanins should not be confused with the MS4A (Membrane-spanning 4 domain) family of proteins. To date, there are more than 30 human tetraspanins, presumably all of which participate in the creation of tetraspanin webs (or multimolecular microdomains) that traverse the cell membrane. As with other superfamily members, CD9 forms homodimers and associates with essentially five classes of molecules: Integrins ( alpha IIb beta 3, alpha 6 beta 4, alpha 3-6 beta 1), IgSF members (ICAM-1, VCAM-1, IgSF8/EWI-2, CD9P1), ectoenzymes (ADAM-12, MT1-MMP) intracellular signaling moluecules (PI-4K, PKC) and others (CD81, CD63, IL-16, pro-HBEGF). The microdomain complexes both recruit and regulate individual molecules. CD9-containing microdomains reportedly promote gamete fusion while inhibiting monocyte fusion. Cells known to express CD9 are many and varied, and include oocytes, regulatory B cells (in mouse, not human), endothelial cells, platelets, monocytes, keratinocytes, mast cells, and myoblasts. The extracellular domain(s) or mouse CD9 share 80% and 88% amino acid sequence identity with human and rat CD9, respectively.
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