Mouse CD9 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Stains mouse CD9-transfected cells in flow cytometry,  but not irrelevant transfectants.
  • Source
    Monoclonal Rat IgG2A Clone # 479608
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with mouse CD9
    Accession # P40240
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD9 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CD9 APC‑conjugated Monoclonal Antibody (Catalog # FAB5218A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD9

CD9 is a 22-25 kDa member of the tetraspanin (TM4SF) superfamily of molecules. Although typology of this family shows a cytoplasmic N- and C-terminus, tetraspanins should not be confused with the MS4A (Membrane-spanning 4 domain) family of proteins. To date, there are more than 30 human tetraspanins, presumably all of which participate in the creation of tetraspanin webs (or multimolecular microdomains) that traverse the cell membrane. As with other superfamily members, CD9 forms homodimers and associates with essentially five classes of molecules: Integrins ( alpha IIb beta 3, alpha 6 beta 4, alpha 3-6 beta 1), IgSF members (ICAM-1, VCAM-1, IgSF8/EWI-2, CD9P1), ectoenzymes (ADAM-12, MT1-MMP) intracellular signaling moluecules (PI-4K, PKC) and others (CD81, CD63, IL-16, pro-HBEGF). The microdomain complexes both recruit and regulate individual molecules. CD9-containing microdomains reportedly promote gamete fusion while inhibiting monocyte fusion. Cells known to express CD9 are many and varied, and include oocytes, regulatory B cells (in mouse, not human), endothelial cells, platelets, monocytes, keratinocytes, mast cells, and myoblasts. The extracellular domain(s) or mouse CD9 share 80% and 88% amino acid sequence identity with human and rat CD9, respectively.

  • Entrez Gene IDs:
    928 (Human); 12527 (Mouse)
  • Alternate Names:
    BA2; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 molecule; CD9; Cell growth-inhibiting gene 2 protein; DRAP-27; Leukocyte antigen MIC3; MIC3; motility related protein-1,5H9 antigen; Motility-related protein; MRP-1FLJ99568; p24; tetraspanin-29; TSPAN29; TSPAN-29; TSPAN29MIC3CD9 antigen (p24)
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse CD9 APC-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information