Mouse CD9 Antibody

Detection of CD9 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CD9 Monoclonal Antibody (Catalog # MAB5218, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG APC-conjugated antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

CD9 in Mouse Kidney. CD9 was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse CD9 Monoclonal Antibody (Catalog # MAB5218) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of CD9 by Western Blot Quantification of A beta -bound and GM1-containing EVs using the idICA. A Western blot analysis of A beta, ganglioside GM1, and beta III tubulin in APP-N2a cell lysates (1 × 105 cells/lane) and EVs (1 × 107 cells/lane). B Representative fluorescent images of various concentrations of APP-N2a-derived EVs in the idICA, which is constructed from CTB capture and anti-A beta detection. Each image displays a block of well array corresponding to 10,000 microwells. Scale bar, 200 μm. C, D The ratio of fluorescent beads to trapped beads in a block of well array is plotted as the concentration of A beta captured on CTB-coated beads (CTB-BAN50) in APP-N2a-derived EVs. Plots on the semi-logarithmic (C) and linear (D) scales are shown. Data represent mean ± SD (n = 3 each). E Representative images of APP-N2a-derived EVs (2500 ng protein) in the double color idICA using anti-CD9 antibody and BAN50. Each image displays a block of well array corresponding to 10,000 microwells. Scale bar, 200 μm. F The ratio of BAN50 or CD9 fluorescent beads to trapped beads. G The overlap rate between BAN50 and CD9 fluorescent beads. Data represent mean ± SD (n = 5 each) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36184615), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CD9 by Western Blot Quantification of GM1-containing EVs Using the idICA. A Western blot analysis of CD9, ganglioside GM1, and beta III tubulin in N2a cell lysates (1 × 105 cells/lane) and EVs (1 × 107 cells/lane). B Representative fluorescent images of various concentrations of N2a-derived EVs in the idICA, which is constructed from CTB capture and anti-CD9 detection. Each image shows a block of well array corresponding to 10,000 microwells. Scale bar, 200 μm. C, D The ratio of fluorescent beads to trapped beads in a block of well array is plotted as the concentration of CD9 captured on CTB-coated beads (CTB-CD9) in N2a-derived EVs. Plots on the semi-logarithmic (C) and linear (D) scales are shown. Data represent mean ± SD (n = 3 each) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36184615), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CD9 by Western Blot Quantification of A beta -bound and GM1-containing EVs using the idICA. A Western blot analysis of A beta, ganglioside GM1, and beta III tubulin in APP-N2a cell lysates (1 × 105 cells/lane) and EVs (1 × 107 cells/lane). B Representative fluorescent images of various concentrations of APP-N2a-derived EVs in the idICA, which is constructed from CTB capture and anti-A beta detection. Each image displays a block of well array corresponding to 10,000 microwells. Scale bar, 200 μm. C, D The ratio of fluorescent beads to trapped beads in a block of well array is plotted as the concentration of A beta captured on CTB-coated beads (CTB-BAN50) in APP-N2a-derived EVs. Plots on the semi-logarithmic (C) and linear (D) scales are shown. Data represent mean ± SD (n = 3 each). E Representative images of APP-N2a-derived EVs (2500 ng protein) in the double color idICA using anti-CD9 antibody and BAN50. Each image displays a block of well array corresponding to 10,000 microwells. Scale bar, 200 μm. F The ratio of BAN50 or CD9 fluorescent beads to trapped beads. G The overlap rate between BAN50 and CD9 fluorescent beads. Data represent mean ± SD (n = 5 each) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36184615), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CD9 by Western Blot Quantification of GM1-containing EVs Using the idICA. A Western blot analysis of CD9, ganglioside GM1, and beta III tubulin in N2a cell lysates (1 × 105 cells/lane) and EVs (1 × 107 cells/lane). B Representative fluorescent images of various concentrations of N2a-derived EVs in the idICA, which is constructed from CTB capture and anti-CD9 detection. Each image shows a block of well array corresponding to 10,000 microwells. Scale bar, 200 μm. C, D The ratio of fluorescent beads to trapped beads in a block of well array is plotted as the concentration of CD9 captured on CTB-coated beads (CTB-CD9) in N2a-derived EVs. Plots on the semi-logarithmic (C) and linear (D) scales are shown. Data represent mean ± SD (n = 3 each) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36184615), licensed under a CC-BY license. Not internally tested by R&D Systems.