Mouse CD97 v2 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) CD97 and no cross-reactivity with recombinant human (rh) CD96v2, rhCD97, or rmCD96 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 587702
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse CD97v2
    Accession # AAH06676
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD97 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD97 v2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB3734G, filled histogram) or isotype control antibody (Catalog # IC005G, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD97
CD97 is an approximately 90-100 kDa, 818 amino acid (aa) N-glycosylated member of the LNB-TM7 family of G protein-coupled proteins. Mouse CD97 contains a 510 aa N-terminal extracellular region with four EGF-like domains and a short 45 aa cytoplasmic tail. It undergoes proteolytic processing between Thr513-Ser514 to generate a 68-80 kDa N-terminal alpha -chain, and a 26-28 kDa membrane-bound beta -chain. The two chains remain non-covalently linked on the cell membrane with dissociation and solubilization of the alpha -chain under select conditions. CD97 in mouse has an alternately spliced variant 2 (V2) that exhibits a 94 aa deletion, resulting in the loss of the third EGF-like repeat (aa 69-119). CD97 is expressed on monocytes, macrophages, neutrophils, T cells, B cell subsets, dendritic cells, mesenchymal stem cells, and smooth muscle cells. It binds CD55/DAF, chondroitin sulfate, CD90, and  alpha V beta 3 Integrin plus alpha 5 beta 1 Integrin. Interaction with these molecules may vary between splice forms of CD97. On the cell surface, the alpha beta  heterodimer may "homodimerize", and form a true heterodimer with the 7-transmembrane LPAR1. CD97 participates in neutrophil migration, host defense, and angiogenesis. It also facilitates chemokine binding to PMNs, and inhibits select cell migration by downregulating MMP activity. Within aa 24-384, mouse CD97 variant 2 shares 51% aa and 76% aa sequence identity with corresponding regions of human and rat CD97, respectively.
  • Entrez Gene IDs:
    976 (Human); 26364 (Mouse)
  • Alternate Names:
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC005G
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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