Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 587702
View all CD97 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD97v2
Gln24-His384
Accession # AAH06676

Specificity

In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) CD97 and no cross-reactivity with recombinant human (rh) CD96v2, rhCD97, or rmCD96 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse CD97 v2 APC‑conjugated Antibody

Detection of CD97 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD97 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD97 v2 APC-conjugated Monoclonal Antibody (Catalog # FAB3734A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CD97 v2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD97

CD97 is an approximately 100 kDa N-glycosylated member of the LNB-TM7 family of G protein-coupled proteins. It contains a 510 amino acid (aa) N-terminal extracellular region with four EGF-like domains and a juxtamembrane GPS motif which enables proteolytic shedding of the N-terminal extracellular domain. The alternately spliced variant 2 of CD97 exhibits a 94 aa deletion that results in loss of the third EGF-like repeat. CD97 is expressed on monocytes, macrophages, T cells, B cell subsets, dendritic cells, hematopoietic progenitor cells, and smooth muscle cells. It binds CD55/DAF, chondroitin sulfate, and Integrin although interaction with these molecules can vary between splice forms of CD97. CD97 participates in neutrophil migration, host defense, and angiogenesis. Within aa 24-384, mouse CD97 variant 2 shares 51% aa and 76% aa sequence identity with corresponding regions of human and rat CD97, respectively.

Alternate Names

CD97

Entrez Gene IDs

976 (Human); 26364 (Mouse)

Gene Symbol

ADGRE5

UniProt

AAH06676

Additional CD97 Products

Product Documents for Mouse CD97 v2 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CD97 v2 APC‑conjugated Antibody

For research use only

Citations for Mouse CD97 v2 APC‑conjugated Antibody

Customer Reviews for Mouse CD97 v2 APC‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse CD97 v2 APC‑conjugated Antibody and earn rewards!

Have you used Mouse CD97 v2 APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies