Mouse CD97 v2 PE-conjugated Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) CD97 and no cross-reactivity with recombinant human (rh) CD96v2, rhCD97, or rmCD96 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 587702
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse CD97v2
    Gln24-His384
    Accession # AAH06676
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD97v2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD97v2 PE‑conjugated Monoclonal Antibody (Catalog # FAB3734P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD97
CD97 is an approximately 100 kDa N-glycosylated member of the LNB-TM7 family of G protein-coupled proteins. It contains a 510 amino acid (aa) N-terminal extracellular region with four EGF-like domains and a juxtamembrane GPS motif which enables proteolytic shedding of the N-terminal extracellular domain. The alternately spliced variant 2 of CD97 exhibits a 94 aa deletion that results in loss of the third EGF-like repeat. CD97 is expressed on monocytes, macrophages, T cells, B cell subsets, dendritic cells, hematopoietic progenitor cells, and smooth muscle cells. It binds CD55/DAF, Chondroitin Sulfate, and Integrin although interaction with these molecules can vary between splice forms of CD97. CD97 participates in neutrophil migration, host defense, and angiogenesis. Within aa 24-384, mouse CD97 variant 2 shares 51% aa and 76% aa sequence identity with corresponding regions of human and rat CD97, respectively.
  • Entrez Gene IDs:
    976 (Human); 26364 (Mouse)
  • Alternate Names:
    CD97
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 PE-conjugated Antibody

Ctrl IC005P 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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