Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 935610
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CEACAM-2
Gln35-Asp422
Accession # Q925P2

Specificity

Detects mouse CEACAM-2 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse CEACAM-2 Antibody

Detection of CEACAM-2 antibody in HEK293 Human Cell Line Transfected with Mouse CEACAM-2 and eGFP antibody by Flow Cytometry.

Detection of CEACAM-2 in HEK293 Human Cell Line Transfected with Mouse CEACAM-2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) mouse CEACAM-2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse CEACAM-2 Monoclonal Antibody (Catalog # MAB9214) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB005). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CEACAM-2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CEACAM-2 and eGFP

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-2

CEACAM2 is a member of the family of carcinoembryonic antigen-related cell adhesion molecules. CEACAM2 controls energy balance and peripheral insulin action. It is involved in the regulation of feeding behavior particularly in the ventromedial nucleus of hypothalamus (VMH) regulation of food intake. Alternatively spliced transcripts encoding different proteins have been described.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 2

Alternate Names

AI267129, Bgp2, CEACAM2, Ceacam2-L

Entrez Gene IDs

26367 (Mouse)

Gene Symbol

CEACAM2

UniProt

Additional CEACAM-2 Products

Product Documents for Mouse CEACAM-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CEACAM-2 Antibody

For research use only

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FAQs

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