Mouse CRISP-1 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY4675-05
Ancillary Products Available
Mouse CRISP-1 ELISA Standard Curve
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse CRISP-1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CRISP-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Mouse CRISP-1 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CRISP-1

Members of the CRISP family contain three conserved domains: a plant pathogenesis related group 1 (PR-1) domain, a short hinge region and a cysteine rich (CR) domain. Some CRISP family members play roles in male fertility, while others may play roles in innate immunity.

Long Name:
Cysteine-rich Secretory Protein 1
Entrez Gene IDs:
167 (Human); 11571 (Mouse); 64827 (Rat)
Alternate Names:
Acidic epididymal glycoprotein homolog; acidic epididymal glycoprotein-like 1; AEGL1; AEG-related protein; ARPAEG-like protein; CRISP1; CRISP-1; CRISP-1AEGL1HSCRISP1D; cysteine-rich secretory protein 1; cysteine-rich secretory protein-1 delta; HSCRISP1G; HUMARP

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Reviews for Mouse CRISP-1 DuoSet ELISA

There are currently no reviews for this product. Be the first to review Mouse CRISP-1 DuoSet ELISA and earn rewards!

Have you used Mouse CRISP-1 DuoSet ELISA?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review