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Mouse CRISP-1 DuoSet ELISA

Catalog #: DY4675-05 Datasheet / COA / SDS

Discontinued Product

DY4675-05 has been discontinued.
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Mouse CRISP-1 ELISA Standard Curve
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse CRISP-1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CRISP-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Mouse CRISP-1 ELISA Standard Curve View Larger

Mouse CRISP-1 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CRISP-1

Members of the CRISP family contain three conserved domains: a plant pathogenesis related group 1 (PR-1) domain, a short hinge region and a cysteine rich (CR) domain. Some CRISP family members play roles in male fertility, while others may play roles in innate immunity.

Long Name:
Cysteine-rich Secretory Protein 1
Entrez Gene IDs:
167 (Human); 11571 (Mouse); 64827 (Rat)
Alternate Names:
Acidic epididymal glycoprotein homolog; acidic epididymal glycoprotein-like 1; AEGL1; AEG-related protein; ARPAEG-like protein; CRISP1; CRISP-1; CRISP-1AEGL1HSCRISP1D; cysteine-rich secretory protein 1; cysteine-rich secretory protein-1 delta; HSCRISP1G; HUMARP

FAQs

No product specific FAQs exist for this product, however you may

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