Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 126315
View all CX3CL1/Fractalkine Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CX3CL1/Fractalkine, S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CX3CL1/Fractalkine, and E. coli-derived recombinant mouse CX3CL1/Fractalkine

Specificity

Detects mouse CX3CL1/Fractalkine in Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with the chemokine domain of recombinant human (rh) CX3CL1, 13% cross-reactivity with rhCX3CL1 (full length), and 50% cross-reactivity with recombinant rat CX3CL1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CX3CL1/Fractalkine APC‑conjugated Antibody

Detection of CX3CL1/Fractalkine antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of CX3CL1/Fractalkine in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse CX3CL1/Fractalkine APC-conjugated Monoclonal Antibody (Catalog # FAB571A, filled histogram) or isotype control antibody (IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CX3CL1/Fractalkine in HEK293 cells transfected with mouse CX3CL1 and eGFP cells by Flow Cytometry.

HEK293 cells transfected with mouse CX3CL1 and eGFP was stained with eGFP and either (A) Rat Anti-Mouse CX3CL1/Fractalkine APC‑conjugated Monoclonal Antibody (Catalog # FAB571A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CX3CL1/Fractalkine APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: bEnd.3 mouse endothelioma cell line and HEK293 cells transfected with mouse CX3CL1 and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CX3CL1/Fractalkine

CX3CL1, also known as Fractalkine, is a type I transmembrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. Although membrane-bound, it can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Alternate Names

FKN, Fractalkine, Neurotactin

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Gene Symbol

CX3CL1

Additional CX3CL1/Fractalkine Products

Product Documents for Mouse CX3CL1/Fractalkine APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CX3CL1/Fractalkine APC‑conjugated Antibody

For research use only

Citations for Mouse CX3CL1/Fractalkine APC‑conjugated Antibody

Customer Reviews for Mouse CX3CL1/Fractalkine APC‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse CX3CL1/Fractalkine APC‑conjugated Antibody and earn rewards!

Have you used Mouse CX3CL1/Fractalkine APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies