Mouse CX3CL1/Fractalkine Chemokine Domain Antibody

Catalog # Availability Size / Price Qty
MAB571-SP
MAB571-100
MAB571-500
Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Mouse CX3CL1/Fractalkine Antibody.
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Mouse CX3CL1/Fractalkine Chemokine Domain Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CX3CL1/Fractalkine in Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with the chemokine domain of recombinant human (rh) CX3CL1, 13% cross-reactivity with rhCX3CL1, and 50% cross-reactivity with recombinant rat CX3CL1.
Source
Monoclonal Rat IgG2A Clone # 126315
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CX3CL1/Fractalkine, S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CX3CL1/Fractalkine, and E. coli-derived recombinant mouse CX3CL1/Fractalkine
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CX3CL1/Fractalkine Full Length (Catalog # 472-FF)
under non-reducing conditions only

Mouse CX3CL1/Fractalkine Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog # BAF472)

Standard: Recombinant Mouse CX3CL1/Fractalkine (Full Length) Protein (Catalog # 472-FF)

Neutralization
Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CX3CR1. The Neutralization Dose (ND50) is typically 0.75-3.5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CX3CL1/Fractalkine aa 25‑105.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Mouse CX3CL1/Fractalkine Antibody. View Larger

Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Mouse CX3CL1/Fractalkine Antibody. Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571). The ND50 is typically 0.75-3.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Entrez Gene IDs
6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names
ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Product Datasheets

Citations for Mouse CX3CL1/Fractalkine Chemokine Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Prevention of lipopolysaccharide-induced preterm labor by the lack of CX3CL1-CX3CR1 interaction in mice
    Authors: M Mizoguchi, Y Ishida, M Nosaka, A Kimura, Y Kuninaka, T Yahata, S Nanjo, S Toujima, S Minami, K Ino, N Mukaida, T Kondo
    PLoS ONE, 2018;13(11):e0207085.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. p16Ink4a and p21Cip1/Waf1 promote tumour growth by enhancing myeloid-derived suppressor cells chemotaxis
    Authors: A Okuma, A Hanyu, S Watanabe, E Hara
    Nat Commun, 2017;8(1):2050.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen
    Authors: Bryan Simons
    J. Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types:
    Applications: Neutralization
  4. Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
    J Neuroinflammation, 2012;9(0):193.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Gene therapy with CX3CL1/Fractalkine induces antitumor immunity to regress effectively mouse hepatocellular carcinoma.
    Authors: Tang L, Hu HD, Hu P, Lan YH, Peng ML, Chen M, Ren H
    Gene Ther., 2007;14(16):1226-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Reviews for Mouse CX3CL1/Fractalkine Chemokine Domain Antibody

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Mouse CX3CL1/Fractalkine Chemokine Domain Antibody
By Anonymous on 06/29/2016
Application: IP Sample Tested: Brain homogenate,Cell culture lysate,Cell culture supernatant Species: Mouse