Mouse CXADR Biotinylated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXADR in Western blots. In Western blots, approximately 35% cross-reactivity with recombinant human CXADR is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CXADR
    Leu20-Gly237
    Accession # P97792
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CXADR Fc Chimera (Catalog # 3724-NR)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXADR

CXADR (coxsackie and adenovirus receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1‑3). CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CXADR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4‑6). The 365 amino acid (aa) mouse CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). A PDZ binding motif at the C‑terminus interacts with several cytoplasmic junctional proteins (1). The ECD of mouse CXADR shares 97%, 90%, 89%, 89% and 88% aa sequence identity with the corresponding regions of rat, human, bovine, porcine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C‑terminal 15 aa shows the same expression pattern, but may show different subcellular localization (4, 8). Transcription of other splice variants has been detected, but not their translation. A secreted form identified in serum and pleural fluid can block viral infection (9).

  • References:
    1. Coyne, C.B. and J.M. Bergelson (2005) Adv. Drug Deliv. Rev. 57:869.
    2. Philipson, L. and R.F. Pettersson (2004) Curr. Top. Microbiol. Immunol. 273:87.
    3. Tomko, R.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3352.
    4. Raschperger, E. et al. (2006) Exp. Cell Res. 312:1566.
    5. Hotta, Y. et al. (2003) Dev. Brain Res. 143:1.
    6. Hauwel, M. et al. (2005) Brain Res. Rev. 48:265.
    7. Chen, J. et al. (2006) Circ. Res. 98:923.
    8. Mirza, M. et al. (2006) Exp. Cell Res. 312:817.
    9. Bernal, R.M. et al. (2002) Clin. Cancer Res. 8:1915.
  • Long Name:
    Coxsackie and Adenovirus Receptor
  • Entrez Gene IDs:
    1525 (Human); 13052 (Mouse)
  • Alternate Names:
    CAR10Coxsackievirus B-adenovirus receptor; CAR4/6; coxsackie virus and adenovirus receptor; coxsackie virus B receptor; coxsackievirus and adenovirus receptor; CVB3 binding protein; CVB3 BP; CVB3-binding protein; CXADR; HCAR; HCVADR
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