Mouse CXADR Antibody AF2654: R&D Systems

Mouse CXADR Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXADR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 35% cross‑reactivity with recombinant human CXADR is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CXADR
    Leu20-Gly237
    Accession # P97792
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Simple Western
    12.5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed frozen sections of mouse embryo (E15.5)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse CXADR by Western Blot. Western blot shows lysates of mouse liver tissue and mouse embryo tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CXADR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CXADR at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse CXADR by Simple WesternTM. Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. Specific bands were detected for CXADR at approximately 60 and 53 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse CXADR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2654) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXADR

CXADR (Coxsackie and Adenovirus Receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1‑3). CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CXADR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4‑6). The 365 amino acid (aa) mouse CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). A PDZ binding motif at the C‑terminus interacts with several cytoplasmic junctional proteins (1). The ECD of mouse CXADR shares 97%, 90%, 89%, 89% and 88% aa sequence identity with the corresponding regions of rat, human, bovine, porcine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C‑terminal 15 aa shows the same expression pattern, but may show different subcellular localization (4, 8). Transcription of other splice variants has been detected, but not their translation. A secreted form identified in serum and pleural fluid can block viral infection (9).

  • References:
    1. Coyne, C.B. and J.M. Bergelson (2005) Adv. Drug Deliv. Rev. 57:869.
    2. Philipson, L. and R.F. Pettersson (2004) Curr. Top. Microbiol. Immunol. 273:87.
    3. Tomko, R.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3352.
    4. Raschperger, E. et al. (2006) Exp. Cell Res. 312:1566.
    5. Hotta, Y. et al. (2003) Dev. Brain Res. 143:1.
    6. Hauwel, M. et al. (2005) Brain Res. Rev. 48:265.
    7. Chen, J. et al. (2006) Circ. Res. 98:923.
    8. Mirza, M. et al. (2006) Exp. Cell Res. 312:817.
    9. Bernal, R.M. et al. (2002) Clin. Cancer Res. 8:1915.
  • Long Name:
    Coxsackie and Adenovirus Receptor
  • Entrez Gene IDs:
    1525 (Human); 13052 (Mouse)
  • Alternate Names:
    CAR10Coxsackievirus B-adenovirus receptor; CAR4/6; coxsackie virus and adenovirus receptor; coxsackie virus B receptor; coxsackievirus and adenovirus receptor; CVB3 binding protein; CVB3 BP; CVB3-binding protein; CXADR; HCAR; HCVADR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Coxsackievirus Adenovirus Receptor Loss Impairs Adult Neurogenesis, Synapse Content, and Hippocampus Plasticity
    J Neurosci, 2016;36(37):9558-71.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  2. A pRb-responsive, RGD-modified, and hyaluronidase-armed canine oncolytic adenovirus for application in veterinary oncology.
    Authors: Laborda E, Puig-Saus C, Rodriguez-Garcia A, Moreno R, Cascallo M, Pastor J, Alemany R
    Mol Ther, 2014;22(5):986-98.
    Species: Canine
    Sample Type: Whole Cells
    Application: Flow
  3. Enhanced adenoviral gene delivery to motor and dorsal root ganglion neurons following injection into demyelinated peripheral nerves.
    Authors: Zhang Y, Zheng Y, Zhang YP
    J. Neurosci. Res., 2010;88(11):2374-84.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
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