Mouse CXCR1/IL-8 RA APC-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB8628A-025
FAB8628A-100
Detection  of CXCR1/IL‑8 RA in HEK293 Human Cell Line Transfected With Mouse CXCR1/IL-8 RA and eGFP by Flow Cytometry.
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Mouse CXCR1/IL-8 RA APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCR1 transfectants but not CXCR2 transfectants in flow cytometry.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1122A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
A peptide corresponding to the N-terminal amino acid sequence of mouse CXCR1
Accession # NP_839972
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of CXCR1/IL‑8 RA in HEK293 Human Cell Line Transfected With Mouse CXCR1/IL-8 RA and eGFP by Flow Cytometry. View Larger

Detection of CXCR1/IL‑8 RA in HEK293 Human Cell Line Transfected With Mouse CXCR1/IL-8 RA and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR1/IL-8 RA or (B) mouse CXCR2/IL-8 RB and eGFP was stained with Rabbit Anti-Mouse CXCR1/IL‑8 RA APC‑conjugated Monoclonal Antibody (Catalog # FAB8628A). Quadrant markers were set based on control antibody staining (Catalog # IC105A). View our protocol for Staining Membrane-associated Proteins

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR1/IL-8 RA

CXCR1 is a G protein-linked seven transmembrane domain spanning chemokine receptor that binds to mouse GCP-2, which is a powerful chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. The chemokine receptors CXCR1 and CXCR2 play an important role in mediating neutrophil recruitment and neutrophil-dependent injury in several models of inflammation.

Long Name
Interleukin 8 Receptor A
Entrez Gene IDs
3577 (Human); 227288 (Mouse); 54258 (Rat)
Alternate Names
CD128; CD181 antigen; CD181; CDw128aC-C; chemokine (C-X-C motif) receptor 1; CKR-1; CMKAR1; C-X-C chemokine receptor type 1; CXCR1; CXC-R1; CXCR-1; High affinity interleukin-8 receptor A; IL-8 receptor type 1; IL-8R A; IL8R1; IL8RA; IL-8RA; IL8RAC-C-CKR-1; IL8RBA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A

Product Datasheets

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