Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1122A
View all CXCR1/IL-8RA Primary Antibodies »

Product Specifications

Immunogen

Mouse CXCR1/IL‑8 RA Peptide
Accession # Q810W6

Specificity

Detects mouse CXCR1 transfectants but not CXCR2 transfectants by flow cytometry.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse CXCR1/IL-8RA Antibody

Detection of CXCR1/IL-8 RA antibody in HEK293 Human Cell Line Transfected with Mouse CXCR1/IL-8 RA and eGFP antibody by Flow Cytometry.

Detection of CXCR1/IL‑8 RA in HEK293 Human Cell Line Transfected with Mouse CXCR1/IL‑8 RA and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR1/IL-8 RA or (B) irrelevant protein was stained with Rabbit Anti-Mouse CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB8628). Quadrant markers were set based on control antibody staining (AB-105-C). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CXCR1/IL-8RA Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample:   HEK293 human embryonic kidney cell line transfected with mouse CXCR1/IL‑8 RA

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR1/IL-8RA

CXCR1, also known as IL-8 RA and CD181, is an approximately 60 kDa 7TM glycoprotein that functions as a receptor for the chemokine CXCL8/IL-8. It is expressed on neutrophils, monocytes, CD8 T cells, FoxP3+ CD4 Treg cells, mast cells, neuronal and glial cells, vascular endothelial cells, and melanoma. CXCR1 forms homodimers and heterodimers with CXCR2/IL-8 RB. It can be cleaved from neutrophils in the lungs of cystic fibrosis patients to release fragments that promote CXCL8 production from airway epithelial cells. CXCR1 mediates neutrophil activation and chemotaxis to sites of inflammation as well as angiogenesis and melanoma invasiveness. Mouse CXCR1 shares 65% and 89% amino acid sequence identity with human and rat CXCR1, respectively.

Long Name

Interleukin 8 Receptor A

Alternate Names

CD181, IL-8 RA, IL8RA

Entrez Gene IDs

3577 (Human); 227288 (Mouse); 54258 (Rat)

Gene Symbol

CXCR1

UniProt

Q810W6

Additional CXCR1/IL-8RA Products

Product Documents for Mouse CXCR1/IL-8RA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CXCR1/IL-8RA Antibody

For research use only

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