Mouse CXCR3 PE-conjugated Antibody

(9 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCR3.
  • Source
    Monoclonal Rat IgG2A Clone # 220803
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    CHO Chinese hamster ovary cell line transfected with mouse CXCR3
    Met1-Leu367
    Accession # AAC40163
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CXCR3 PE‑conjugated Monoclonal Antibody (Catalog # FAB1685P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR3

CXCR3 is a G protein coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10) and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells and NK cells.

  • Entrez Gene IDs:
    2833 (Human); 12766 (Mouse); 84475 (Rat)
  • Alternate Names:
    CD183 antigen; CD183; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C) receptor 3; CKR-L2IP-10 receptor; CMKAR3; C-X-C chemokine receptor type 3; CXCR3; CXC-R3; CXCR-3; G protein-coupled receptor 9CD182; GPR9; GPR9Mig-R; Interferon-inducible protein 10 receptor; IP10 receptor; IP10-R; Mig receptor; MigR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. CXCR4 expression on pathogenic T cells facilitates their bone marrow infiltration in a mouse model of aplastic anemia.
    Authors: Arieta Kuksin C, Gonzalez-Perez G, Minter L
    Blood, 2015;125(13):2087-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Thymic self-reactivity selects natural interleukin 17-producing T cells that can regulate peripheral inflammation.
    Authors: Marks BR, Nowyhed HN, Choi JY, Poholek AC, Odegard JM, Flavell RA, Craft J
    Nat. Immunol., 2009;10(10):1125-32.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Dendritic cells in islets of Langerhans constitutively present beta cell-derived peptides bound to their class II MHC molecules.
    Authors: Calderon B, Suri A, Miller MJ
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(16):6121-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Expression of CXCL4 in microglia in vitro and in vivo and its possible signaling through CXCR3.
    Authors: de Jong EK, de Haas AH, Brouwer N, van Weering HR, Hensens M, Bechmann I, Pratley P, Wesseling E, Boddeke HW, Biber K
    J. Neurochem., 2008;105(5):1726-36.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Both Th1 and Th17 are immunopathogenic but differ in other key biological activities.
    Authors: Cox CA, Shi G, Yin H, Vistica BP, Wawrousek EF, Chan CC, Gery I
    J. Immunol., 2008;180(11):7414-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. A systematic methodology for proteome-wide identification of peptides inhibiting the proliferation and migration of endothelial cells.
    Authors: Karagiannis ED, Popel AS
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(37):13775-80.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Amelioration of delayed-type hypersensitivity responses by IL-27 administration.
    Authors: Miyazaki Y, Shimanoe Y, Wang S, Yoshida H
    Biochem. Biophys. Res. Commun., 2008;373(3):397-402.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  8. E- and P-selectins synergistically inhibit bleomycin-induced pulmonary fibrosis.
    Authors: Horikawa M, Fujimoto M, Hasegawa M, Matsushita T, Hamaguchi Y, Kawasuji A, Matsushita Y, Fujita T, Ogawa F, Takehara K, Steeber DA, Sato S
    Am. J. Pathol., 2006;169(3):740-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. CXCL10 is the key ligand for CXCR3 on CD8+ effector T cells involved in immune surveillance of the lymphocytic choriomeningitis virus-infected central nervous system.
    Authors: Christensen JE, de Lemos C, Moos T, Christensen JP, Thomsen AR
    J. Immunol., 2006;176(7):4235-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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