Mouse CXCR6 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2145R-100UG
Detection of CXCR6 in BaF3 mouse cell line transfected with mouse CXCR6 and eGFP by Flow Cytometry.
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Mouse CXCR6 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Stains mouse CXCR6-transfected cells but not irrelevant transfectants.
Source
Monoclonal Rat IgG2B Clone # 221002
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with mouse CXCR6
Met1-Leu351
Accession # Q9EQ16
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CXCR6 in BaF3 mouse cell line transfected with mouse CXCR6 and eGFP by Flow Cytometry. View Larger

Detection of CXCR6 in BaF3 mouse cell line transfected with mouse CXCR6 and eGFP by Flow Cytometry. BaF3 mouse pro-B cell line transfected with mouse CXCR6 and eGFP was stained with and either (A) Rat Anti-Mouse CXCR6 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB2145R) or (B) Rat IgG2B Alexa Fluor 647 Isotype Control (Catalog # IC013R). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Entrez Gene IDs
10663 (Human); 80901 (Mouse); 100124593 (Rat)
Alternate Names
Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186

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