Mouse CXCR6 PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Stains mouse CXCR6-transfected cells but not irrelevant transfectants.
  • Source
    Monoclonal Rat IgG2B Clone # 221002
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    CHO Chinese hamster ovary cell line transfected with mouse CXCR6
    Met1-Leu351
    Accession # Q9EQ16
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR6 in BaF3 Mouse Cell Line Transfected with Mouse CXCR6 and eGFP by Flow Cytometry. BaF3 mouse pro-B cell line transfected with mouse CXCR6 and eGFP was stained with and either (A) Rat Anti-Mouse CXCR6 PE‑conjugated Monoclonal Antibody (Catalog # FAB2145P) or (B) Rat IgG2B Phycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with PMA and Calcium Ionomycin overnight were stained with Rat Anti-Mouse CXCR6 PE‑conjugated Monoclonal Antibody (Catalog # FAB2145P) and Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

  • Entrez Gene IDs:
    10663 (Human); 80901 (Mouse); 100124593 (Rat)
  • Alternate Names:
    Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Professional memory CD4+ T lymphocytes preferentially reside and rest in the bone marrow.
    Authors: Tokoyoda K, Zehentmeier S, Hegazy AN, Albrecht I, Grun JR, Lohning M, Radbruch A
    Immunity, 2009;30(5):721-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006;118(9):2147-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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