Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 221002
View all CXCR6 Primary Antibodies »

Product Specifications

Immunogen

CHO Chinese hamster ovary cell line transfected with mouse CXCR6
Met1-Leu351
Accession # Q9EQ16

Specificity

Stains mouse CXCR6-transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CXCR6 Antibody

Detection of CXCR6 antibody in BaF3 Mouse Cell Line Transfected with Mouse CXCR6 and eGFP antibody by Flow Cytometry.

Detection of CXCR6 in BaF3 Mouse Cell Line Transfected with Mouse CXCR6 and eGFP by Flow Cytometry.

BaF3 mouse pro-B cell line transfected with mouse CXCR6 and eGFP was stained with and either (A) Rat Anti-Mouse CXCR6 Monoclonal Antibody (Catalog # MAB2145) or (B) Rat IgG2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CXCR6 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: BaF3 mouse pro-B cell line transfected with mouse CXCR6 and eGFP

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Alternate Names

Bonzo, CD186, CXCR6, STRL33

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Gene Symbol

CXCR6

UniProt

Q9EQ16

Additional CXCR6 Products

Product Documents for Mouse CXCR6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CXCR6 Antibody

For research use only

Citations for Mouse CXCR6 Antibody

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