Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgM Clone # 734110
View all CXCR7/RDC-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCR7/RDC-1 extracellular N-terminus and loops
Met1-Leu47, Ser103-Lys118, Lys184-Glu213, Leu274-Ala296
Accession # P56485

Specificity

Detects mouse CXCR7/RDC-1 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgM

Scientific Data Images for Mouse CXCR7/RDC‑1 Antibody

Detection of CXCR7 antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of CXCR7 in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB7167, filled histo-gram) or isotype control antibody rat IgM (open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgM Secondary Antibody.
Detection of CXCR7 antibody in HEK293 Human Cell Line Transfected with eGFP and Mouse CXCR7/RDC-1 antibody by Flow Cytometry.

Detection of CXCR7 in HEK293 Human Cell Line Transfected with eGFP and Mouse CXCR7/RDC-1 by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with eGFP and Mouse CXCR7/RDC-1 were stained with Rat Anti-Mouse CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB7167) followed by Allophycocyanin-conjugated-Anti-Rat IgM secondary antibody. Quadrant markers were set based on control antibody staining.

Applications for Mouse CXCR7/RDC‑1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: D3 mouse embryonic stem cell line and HEK293 human embryonic kidney cell line transfected with eGFP and Mouse CXCR7/RDC-1

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR7/RDC-1

CXCR7 (CXC chemokine receptor 7; also GPRN1, RDC-1 and chemokine orphan receptor 1) is a 60 kDa member of the G-protein coupled receptor 1 family. It is expressed on multiple cell types, including neurons, T cells, NK cells, neutrophils, B cells plus angiogenic endothelial cells. CXCR7 forms both homodimers and heterodimers with CXCR4. It selectively binds I-TAC and SDF1, and appears to involve beta -arrestin2 during signaling. Notably, a CXCR7:CXCR4 heterodimer shows increased responsiveness to SDF1, and I-TAC may actually block some SDF1-mediated migration activity. Mouse CXCR7 is a 7-transmembrane glycoprotein that is 362 amino acids (aa) in length. It contains a 47 aa N-terminal extracellular region plus a 43 aa C-terminal cytoplasmic domain. Over the collective extracellular domains (aa 1-47, 103-118, 184-213 and 274-296), mouse CXCR7 shares 97% and 91% aa identity with rat and human CXCR7, respectively.

Alternate Names

ACKR3, CMKOR1, CXCR7, GPR159, RDC-1

Entrez Gene IDs

57007 (Human); 12778 (Mouse); 84348 (Rat)

Gene Symbol

ACKR3

UniProt

P56485

Additional CXCR7/RDC-1 Products

Product Documents for Mouse CXCR7/RDC‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CXCR7/RDC‑1 Antibody

For research use only

Citations for Mouse CXCR7/RDC‑1 Antibody

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