Mouse DNAM-1/CD226 Alexa Fluor® 405-conjugated Antibody

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FAB4436V-100UG
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Mouse DNAM-1/CD226 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DNAM-1/CD226 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DNAM-1/CD226 is observed.
Source
Monoclonal Rat IgG2b Clone # 838216
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DNAM-1/CD226
Met1-Pro254
Accession # Q8K4F0
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse DNAM has a 236 amino acid (aa) extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a 58 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, mouse DNAM-1 shares 52% and 86% aa sequence identity with human and rat DNAM-1, respectively. Additional cDNA transcripts of mouse DNAM-1 may give rise to secreted or transmembrane isoforms with ECD deletions. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112 (2‑4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (3‑7), induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation; conversely, it inhibits the formation of osteoclasts (8‑11). Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung (12). On activated, but not resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to tyrosine and serine phosphorylation of DNAM-1 during activation (7, 10, 13‑15).

References
  1. Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
  2. Tahara-Hanaoka, S. et al. (2005) Biochem. Biophys. Res. Commun. 329:996.
  3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  5. Shibuya, A. et al. (1996) Immunity 4:573.
  6. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
  7. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
  8. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  9. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
  10. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  11. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  12. Morimoto, K. et al. (2007) Oncogene 27:264.
  13. Shibuya, K. et al. (1999) Immunity 11:615.
  14. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
  15. Shibuya, A. et al. (1998) J. Immunol. 166:1671.
Long Name
DNAX Accessory Molecule 1
Entrez Gene IDs
10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
Alternate Names
CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1

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