Mouse DNAM-1/CD226 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse DNAM-1/CD226 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DNAM-1/CD226 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 838216
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse DNAM-1/CD226
    Met1-Pro254
    Accession # Q8K4F0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of DNAM-1/CD226 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD8 alpha APC-conjugated Monoclonal Antibody (Catalog # FAB116A) and either (A) Rat Anti-Mouse DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB4436) or (B) Rat IgG2B Isotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse DNAM has a 236 amino acid (aa) extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a 58 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, mouse DNAM-1 shares 52% and 86% aa sequence identity with human and rat DNAM-1, respectively. Additional cDNA transcripts of mouse DNAM-1 may give rise to secreted or transmembrane isoforms with ECD deletions. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112 (2‑4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (3‑7), induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation; conversely, it inhibits the formation of osteoclasts (8‑11). Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung (12). On activated, but not resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to tyrosine and serine phosphorylation of DNAM-1 during activation (7, 10, 13‑15).

  • References:
    1. Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
    2. Tahara-Hanaoka, S. et al. (2005) Biochem. Biophys. Res. Commun. 329:996.
    3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
    4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
    5. Shibuya, A. et al. (1996) Immunity 4:573.
    6. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
    7. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
    8. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
    9. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
    10. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
    11. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
    12. Morimoto, K. et al. (2007) Oncogene 27:264.
    13. Shibuya, K. et al. (1999) Immunity 11:615.
    14. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
    15. Shibuya, A. et al. (1998) J. Immunol. 166:1671.
  • Long Name:
    DNAX Accessory Molecule 1
  • Entrez Gene IDs:
    10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
  • Alternate Names:
    CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Rat IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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