Mouse DNAM-1/CD226 APC‑conjugated Antibody

R&D Systems | Catalog # FAB4436A

R&D Systems
Discontinued Product
FAB4436A has been discontinued. View all DNAM-1/CD226 products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 838216
View all DNAM-1/CD226 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DNAM-1/CD226
Met1-Pro254
Accession # Q8K4F0

Specificity

Detects mouse DNAM-1/CD226 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DNAM-1/CD226 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse DNAM-1/CD226 APC‑conjugated Antibody

Detection of DNAM-1/CD226 antibody in Mouse Splenoctyes antibody by Flow Cytometry.

Detection of DNAM-1/CD226 in Mouse Splenoctyes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD8a PE-conjugated Monoclonal Antibody (Catalog # FAB116P) and either (A) Rat Anti-Mouse DNAM-1/CD226 APC-conjugated Monoclonal Antibody (Catalog # FAB4436A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse DNAM-1/CD226 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 63-65 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. Mature mouse DNAM has a 236 amino acid (aa) extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a 58 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. Within the ECD, mouse DNAM-1 shares 52% and 86% aa sequence identity with human and rat DNAM-1, respectively. Additional cDNA transcripts of mouse DNAM-1 may give rise to secreted or transmembrane isoforms with ECD deletions. DNAM-1 is expressed on several lymphoid and myeloid cell types including NK cells, CD8+ T cells, CD4+ Th1 cells, NKT cells, monocytes, mast cells and plateletes and interacts with CD155/PVR and Nectin-2/CD112 in trans, and with LFA-1 in cis. Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells, induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation; conversely, it inhibits the formation of osteoclasts. Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung. On activated, but not resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to tyrosine and serine phosphorylation of DNAM-1 during activation. Finally, DNAM-1 is now known to differentiate between DNAM-1+ NK cells that produce inflammatory cytokines, an DNAM-1- NK cells that secrete MIP-1 chemokines.

Long Name

DNAX Accessory Molecule 1

Alternate Names

CD226, DNAM1, PTA1, TLiSA1

Entrez Gene IDs

10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)

Gene Symbol

CD226

UniProt

Q8K4F0

Additional DNAM-1/CD226 Products

Product Documents for Mouse DNAM-1/CD226 APC‑conjugated Antibody

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Product Specific Notices for Mouse DNAM-1/CD226 APC‑conjugated Antibody

For research use only

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