Mouse DNAM-1/CD226 PE-conjugated Antibody

FAB4436P has been discontinued. View all DNAM-1/CD226 products.
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse DNAM-1/CD226 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DNAM-1/CD226 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 838216
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse DNAM-1/CD226
    Met1-Pro254
    Accession # Q8K4F0
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of DNAM-1/CD226 in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD8 alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB116A) and either (A) Rat Anti-Mouse DNAM-1/CD226 PE‑conjugated Monoclonal Antibody (Catalog # FAB4436P) or (B) Rat IgG2B Phycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DNAM-1/CD226
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 63-65 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. Mature mouse DNAM has a 236 amino acid (aa) extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a 58 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. Within the ECD, mouse DNAM-1 shares 52% and 86% aa sequence identity with human and rat DNAM-1, respectively. Additional cDNA transcripts of mouse DNAM-1 may give rise to secreted or transmembrane isoforms with ECD deletions. DNAM-1 is expressed on several lymphoid and myeloid cell types including NK cells, CD8+ T cells, CD4+ Th1 cells, NKT cells, monocytes, mast cells and plateletes and interacts with CD155/PVR and Nectin-2/CD112 in trans, and with LFA-1 in cis. Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells, induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation; conversely, it inhibits the formation of osteoclasts. Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung. On activated, but not resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to tyrosine and serine phosphorylation of DNAM-1 during activation. Finally, DNAM-1 is now known to differentiate between DNAM-1+ NK cells that produce inflammatory cytokines, an DNAM-1- NK cells that secrete MIP-1 chemokines.
  • Long Name:
    DNAX Accessory Molecule 1
  • Entrez Gene IDs:
    10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
  • Alternate Names:
    CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1
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