Mouse E-Selectin/CD62E Antibody
Mouse E-Selectin/CD62E Antibody Summary
Trp22-Pro557
Accession # Q3U5F6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
E-Selectin/CD62E in Mouse Thymus. E-Selectin/CD62E was detected in perfusion fixed frozen sections of mouse thymus using Mouse E-Selectin/CD62E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF575) at 1.7 µg/mL overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin/CD62E
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, or CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E‑Selectin on healthy and diseased tissue. The human and mouse
E‑Selectin proteins share 81% amino acid similarity.
E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.
ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.
Product Datasheets
Citations for Mouse E-Selectin/CD62E Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
6
Citations: Showing 1 - 6
Filter your results:
Filter by:
-
Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis
Authors: S Colijn, V Muthukumar, J Xie, S Gao, CT Griffin
Dis Model Mech, 2020;13(1):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A
PLoS ONE, 2015;10(3):e0119661.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis.
Authors: Roelofs JJ, Teske GJ, Bonta PI, de Vries CJ, Meijers JC, Weening JJ, van der Poll T, Florquin S
Kidney Int., 2009;75(1):52-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation.
Authors: Leeper NJ, Tedesco MM, Kojima Y, Schultz GM, Kundu RK, Ashley EA, Tsao PS, Dalman RL, Quertermous T
Am. J. Physiol. Heart Circ. Physiol., 2009;296(5):H1329-35.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Authors: Kato N, Yuzawa Y, Kosugi T
J. Am. Soc. Nephrol., 2009;20(7):1565-76.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Selective inhibition of proprotein convertases represses the metastatic potential of human colorectal tumor cells.
Authors: Scamuffa N, Siegfried G, Bontemps Y, Ma L, Basak A, Cherel G, Calvo F, Seidah NG, Khatib AM
J. Clin. Invest., 2008;118(1):352-63.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Mouse E-Selectin/CD62E Antibody
There are currently no reviews for this product. Be the first to review Mouse E-Selectin/CD62E Antibody and earn rewards!
Have you used Mouse E-Selectin/CD62E Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
