Mouse E-Selectin/CD62E Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse E-Selectin/CD62E in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human E-Selectin, recombinant mouse (rm) L-Selectin, and rmP-Selectin is observed.
  • Source
    Polyclonal Chicken IgY
  • Purification
    Antigen Affinity-purified from egg yolks
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse E-Selectin/CD62E
    Trp22-Pro557
    Accession # Q3U5F6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse E-Selectin/CD62E Fc Chimera (Catalog # 575-ES)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
E-Selectin/CD62E in Mouse Thymus.
E-Selectin/CD62E was detected in perfusion fixed frozen sections of mouse thymus using Mouse E-Selectin/CD62E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF575) at 1.7 µg/mL overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, or CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E‑Selectin on healthy and diseased tissue. The human and mouse
E‑Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

  • Entrez Gene IDs:
    6401 (Human); 20339 (Mouse); 25544 (Rat)
  • Alternate Names:
    CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
    Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A
    PLoS ONE, 2015;10(3):e0119661.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis.
    Authors: Roelofs JJ, Teske GJ, Bonta PI, de Vries CJ, Meijers JC, Weening JJ, van der Poll T, Florquin S
    Kidney Int., 2009;75(1):52-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation.
    Authors: Leeper NJ, Tedesco MM, Kojima Y, Schultz GM, Kundu RK, Ashley EA, Tsao PS, Dalman RL, Quertermous T
    Am. J. Physiol. Heart Circ. Physiol., 2009;296(5):H1329-35.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009;20(7):1565-76.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Selective inhibition of proprotein convertases represses the metastatic potential of human colorectal tumor cells.
    Authors: Scamuffa N, Siegfried G, Bontemps Y, Ma L, Basak A, Cherel G, Calvo F, Seidah NG, Khatib AM
    J. Clin. Invest., 2008;118(1):352-63.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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Isotype Controls
Description Application Cat# Citations Images  

Normal Chicken IgY Control

Ctrl AB-101-C 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat Anti-Chicken IgY NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL016 1
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Goat Anti-Chicken IgY NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL017 1
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Goat Anti-Chicken IgY Antibody

WB AF010 1
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Goat Anti-Chicken IgY NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL018
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Goat Anti-Chicken IgY Biotinylated Antibody

WB BAF010
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