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Mouse E-Selectin/CD62E Antibody

Catalog #: AF575
10 Citations Datasheet / COA / SDS

Discontinued Product

AF575 has been discontinued.
View all E-Selectin/CD62E products.
E-Selectin/CD62E in Mouse Thymus.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse E-Selectin/CD62E Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse E-Selectin/CD62E in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human E-Selectin and recombinant rat E-Selectin is observed.
Source
Polyclonal Chicken IgY
Purification
Antigen Affinity-purified from egg yolks
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse E-Selectin/CD62E
Trp22-Pro557
Accession # Q3U5F6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse E-Selectin/CD62E Fc Chimera (Catalog # 575-ES)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry E-Selectin/CD62E antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

E-Selectin/CD62E in Mouse Thymus. E-Selectin/CD62E was detected in perfusion fixed frozen sections of mouse thymus using Mouse E-Selectin/CD62E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF575) at 1.7 µg/mL overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, or CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E‑Selectin on healthy and diseased tissue. The human and mouse
E‑Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Entrez Gene IDs
6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)
Alternate Names
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

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Citations for Mouse E-Selectin/CD62E Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammation
    Authors: Hinami Asano, Sanae Hasegawa-Ishii, Ken Arae, Aki Obara, Geoffroy Laumet, Robert Dantzer et al.
    Journal of Neuroimmunology
  2. Carfilzomib Delivery by Quinic Acid-Conjugated Nanoparticles: Discrepancy Between Tumoral Drug Accumulation and Anticancer Efficacy in a Murine 4T1 Orthotopic Breast Cancer Model
    Authors: Yearin Jun, Jun Xu, Hyungjun Kim, Ji Eun Park, Yoo-Seong Jeong, Jee Sun Min et al.
    Journal of Pharmaceutical Sciences
  3. Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis
    Authors: S Colijn, V Muthukumar, J Xie, S Gao, CT Griffin
    Dis Model Mech, 2020-01-24;13(1):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  4. Quinic Acid‐Conjugated Nanoparticles Enhance Drug Delivery to Solid Tumors via Interactions with Endothelial Selectins
    Authors: Jun Xu, Steve Seung‐Young Seung-Young Lee, Howon Seo, Liang Pang, Yearin Jun, Ruo‐Yu Zhang et al.
    Small
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  5. Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
    Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A
    PLoS ONE, 2015-03-23;10(3):e0119661.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009-05-14;20(7):1565-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation.
    Authors: Leeper NJ, Tedesco MM, Kojima Y, Schultz GM, Kundu RK, Ashley EA, Tsao PS, Dalman RL, Quertermous T
    Am. J. Physiol. Heart Circ. Physiol., 2009-03-20;296(5):H1329-35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis.
    Authors: Roelofs JJ, Teske GJ, Bonta PI, de Vries CJ, Meijers JC, Weening JJ, van der Poll T, Florquin S
    Kidney Int., 2008-09-17;75(1):52-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Selective inhibition of proprotein convertases represses the metastatic potential of human colorectal tumor cells.
    Authors: Scamuffa N, Siegfried G, Bontemps Y, Ma L, Basak A, Cherel G, Calvo F, Seidah NG, Khatib AM
    J. Clin. Invest., 2008-01-01;118(1):352-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Quinic Acid‐Conjugated Nanoparticles Enhance Drug Delivery to Solid Tumors via Interactions with Endothelial Selectins
    Authors: Jun Xu, Steve Seung‐Young Seung-Young Lee, Howon Seo, Liang Pang, Yearin Jun, Ruo‐Yu Zhang et al.
    Small

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