Mouse EMMPRIN/CD147 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF772
Detection of EMMPRIN/CD147 by Flow Cytometry
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Product Details
Citations (3)
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Mouse EMMPRIN/CD147 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EMMPRIN in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human EMMPRIN  is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EMMPRIN/CD147
Ala22-Arg209
Accession # NP_001070652
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse EMMPRIN Fc Chimera
Flow Cytometry
2.5 µg/106 cells
Mouse thymocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of EMMPRIN/CD147 by Flow Cytometry View Larger

Detection of EMMPRIN/CD147 by Flow Cytometry Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of EMMPRIN/CD147 by Flow Cytometry View Larger

Detection of EMMPRIN/CD147 by Flow Cytometry Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of EMMPRIN/CD147 by Flow Cytometry View Larger

Detection of EMMPRIN/CD147 by Flow Cytometry Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of EMMPRIN/CD147 by Flow Cytometry View Larger

Detection of EMMPRIN/CD147 by Flow Cytometry Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of EMMPRIN/CD147 by Flow Cytometry View Larger

Detection of EMMPRIN/CD147 by Flow Cytometry Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of EMMPRIN/CD147 by Flow Cytometry View Larger

Detection of EMMPRIN/CD147 by Flow Cytometry Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of EMMPRIN/CD147 by Flow Cytometry View Larger

Detection of EMMPRIN/CD147 by Flow Cytometry Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of EMMPRIN/CD147 by Flow Cytometry View Larger

Detection of EMMPRIN/CD147 by Flow Cytometry Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMMPRIN/CD147

EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.

Long Name
Extracellular Matrix Metalloproteinase Inducer
Entrez Gene IDs
682 (Human); 12215 (Mouse); 102121721 (Cynomolgus Monkey)
Alternate Names
5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor

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Citations for Mouse EMMPRIN/CD147 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Development of a novel human CD147 knock-in NSG mouse model to test SARS-CoV-2 viral infection.
    Authors: Badeti S, Jiang Q, Naghizadeh A, Tseng H, Bushkin Y, Marras S, Nisa A, Tyagi S, Chen F, Romanienko P, Yehia G, Evans D, Lopez-Gonzalez M, Alland D, Russo R, Gause W, Shi L, Liu D
    Cell Biosci, 2022-06-11;12(1):88.
    Species: Transgenic Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  2. Development of a Novel Human CD147 Knock-in NSG Mouse Model to Test SARS-CoV-2 Viral Infection
    Authors: S Badeti, Q Jiang, A Naghizadeh, HC Tseng, Y Bushkin, SAE Marras, A Nisa, S Tyagi, F Chen, P Romanienko, G Yehia, D Evans, M Lopez-Gonz, D Alland, R Russo, W Gause, L Shi, D Liu
    Research square, 2022-04-20;0(0):.
    Species: Transgenic Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  3. Development of a Novel Human CD147 Transgenic NSG Mouse Model to test SARS-CoV-2 Infection and Immune Responses
    Authors: S Badeti, HC Tseng, P Romanienko, G Yehia, D Liu
    Research square, 2021-04-07;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Flow Cytometry, IHC

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