Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all EMMPRIN/CD147 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EMMPRIN/CD147
Ala22-Arg209
Accession # NP_001070652

Specificity

Detects mouse EMMPRIN/CD147 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human EMMPRIN  is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse EMMPRIN/CD147 Biotinylated Antibody

Detection of EMMPRIN/CD147 by Flow Cytometry

Detection of EMMPRIN/CD147 by Flow Cytometry

Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry

Detection of EMMPRIN/CD147 by Flow Cytometry

Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry

Detection of EMMPRIN/CD147 by Flow Cytometry

Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry

Detection of EMMPRIN/CD147 by Flow Cytometry

Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry

Detection of EMMPRIN/CD147 by Flow Cytometry

Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry

Detection of EMMPRIN/CD147 by Flow Cytometry

Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry

Detection of EMMPRIN/CD147 by Flow Cytometry

Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry

Detection of EMMPRIN/CD147 by Flow Cytometry

Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse EMMPRIN/CD147 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse thymocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse EMMPRIN/CD147 Fc Chimera

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EMMPRIN/CD147

EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Entrez Gene IDs

682 (Human); 12215 (Mouse); 102121721 (Cynomolgus Monkey)

Gene Symbol

BSG

UniProt

NP_001070652

Additional EMMPRIN/CD147 Products

Product Documents for Mouse EMMPRIN/CD147 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse EMMPRIN/CD147 Biotinylated Antibody

For research use only

Citations for Mouse EMMPRIN/CD147 Biotinylated Antibody

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