Detection of EpCAM/TROP‑1 in 4T1 Mouse Cell Line by Flow Cytometry.
4T1 mouse breast cancer cell line was stained with Rat Anti-Mouse EpCAM/TROP‑1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8998R, filled histogram) or isotype control antibody (Catalog # IC006R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Epithelial Cellular Adhesion
Molecule (EpCAM), also known as ECD326 and TROP-1 is a 36-41 kDa member of the TROP family of meolcules (1, 2). It is a type I transmembrane glycoprotein that is found on embryonic stem cells and adult non-squamous epithelium such as (respiratory) psuedostratified, (mammary) cuboid, and (colon) simple columnar epithelium. In mouse, the molecule has also been reported on T cells, Langerhans cells and thymic epithelium. Mature mouse EpCAM is 292 amino acids (aa) in length. It possesses a 243 aa extracellular region that contains one thyroglobulin type I domain. Such domains are associated with cathepsin binding and inhibition (2). On the cell surface, EpCAM is reported to participate in TEM (Tetraspanin-enriched microdomain) formation in concert with CD44v6, CD9, ADAM10, Claudin-7 and TS8. It is also noted to form homodimers in-cis and homotetramers in-trans. Although this would suggest the EpCAM acts as an adhesion molecule, in effect it does not, instead acting as a disruptor of the E-Cadherin:cytoskeleton interaction, and thus promoting cell migration (1, 2). The extracellular domain (ECD) of EpCAM undergoes multiple cleavages, principally between Ser200 and Ala210 (3). Cleavage at the Arg80-Arg81 site does not generate a soluble fragment, but rather a disulfide-linked "heterodimer" (3). Cleavage after Gly264 in the juxtamembrane region is followed by secretase cleavage of the transmembrane fragment, generating an ICD that translocates to the nucleus (4). The ECD of mouse EpCAM shares 81% and 90% aa sequence identity with human and rat EpCAM, respectively.
Schnell, U. et al. (2013) Biochim. Biophys. Acta 1828:1989.
Martowicz, A. et al. (2015) Histol. Histopathol. Oct 23:11678 [ePub ahead of print].
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