Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EphA1
Glu27-Glu548
Accession # AAH71215

Specificity

Detects mouse EphA1 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human EphA1 and less than 5% cross-reactivity with recombinant mouse (rm) EphA4, rmEphA6, and rmEphA7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse EphA1 Alexa Fluor® 405‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: EphA1

EphA1, also known as Eph and Esk, is a member of the Eph receptor tyrosine kinase family and binds several Ephrin-A ligands. The A and B class Eph proteins share a common structural organization (1-4). The mouse EphA1 cDNA encodes a 977 amino acid (aa) precursor that includes a 24 aa signal sequence, a 524 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 408 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5, 6). Within the ECD, mouse EphA1 shares 84% aa sequence identity with human EphA1, approximately 40% aa sequence identity with mouse EphA2, 3, 4, 6, 7, and 8, and 27% aa sequence identity with mouse EphA5. A splice variant of mouse EphA1 lacks the transmembrane segment and is predicted to exist as a soluble molecule (7). Membrane bound or clustered Ephrin ligands interact with EphA1 and activate its kinase domain which is capable of Ser, Thr, and Tyr phosphorylation (7). Reverse signaling is propagated through the Ephrin ligand. EphA1 is widely expressed in differentiated epithelial cells, particularly in bone marrow, spleen, thymus, and testes (5, 7). It is expressed on CD4+ T cells but not on CD19+ B cells (8). On T cells, EphA1 induces Tyr phosphorylation of PYK2 and promotes chemokine-induced chemotaxis (8). EphA1 is upregulated or downregulated in a variety of human carcinomas and is implicated in tumor invasiveness (2, 9, 10).

Alternate Names

Esk

Entrez Gene IDs

2041 (Human); 13835 (Mouse)

Gene Symbol

EPHA1

UniProt

Additional EphA1 Products

Product Documents for Mouse EphA1 Alexa Fluor® 405‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse EphA1 Alexa Fluor® 405‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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Protocols

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