Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, ELISA Development (Capture)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-A1
Asp19-Ser182
Accession # P52793

Specificity

Detects mouse Ephrin-A1 in Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Ephrin-A5 is observed and less than 5% cross-reactivity with recombinant mouse (rm) Ephrin-A2, rmEphrin-B1, rmEphrin-B2, rhEphrin-B3, rhEphrin-A3, and rmEphrin-A4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Ephrin‑A1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Ephrin-A1 Fc Chimera (Catalog # 602-A1)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-A1

Janus Kinase 1 (Jak1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak1 is required for the activation of STAT1 and STAT2 in response to interferon alpha.

Alternate Names

B61, EFL-1, EFNA1, EphrinA1, LERK1

Entrez Gene IDs

1942 (Human); 13636 (Mouse)

Gene Symbol

EFNA1

UniProt

Additional Ephrin-A1 Products

Product Documents for Mouse Ephrin‑A1 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Ephrin‑A1 Biotinylated Antibody

For research use only

Related Research Areas

Citations for Mouse Ephrin‑A1 Biotinylated Antibody

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FAQs

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