Janus Kinase 1 (Jak1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak1 is required for the activation of STAT1 and STAT2 in response to interferon alpha.
Mouse Ephrin‑A1 Biotinylated Antibody
R&D Systems | Catalog # BAF602
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, ELISA Development (Capture)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-A1
Asp19-Ser182
Accession # P52793
Asp19-Ser182
Accession # P52793
Specificity
Detects mouse Ephrin-A1 in Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Ephrin-A5 is observed and less than 5% cross-reactivity with recombinant mouse (rm) Ephrin-A2, rmEphrin-B1, rmEphrin-B2, rhEphrin-B3, rhEphrin-A3, and rmEphrin-A4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Ephrin‑A1 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Ephrin-A1 Fc Chimera (Catalog # 602-A1)
Sample: Recombinant Mouse Ephrin-A1 Fc Chimera (Catalog # 602-A1)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Ephrin-A1
Alternate Names
B61, EFL-1, EFNA1, EphrinA1, LERK1
Gene Symbol
EFNA1
UniProt
Additional Ephrin-A1 Products
Product Documents for Mouse Ephrin‑A1 Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Ephrin‑A1 Biotinylated Antibody
For research use only
Related Research Areas
Citations for Mouse Ephrin‑A1 Biotinylated Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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