Mouse ESAM Antibody [Biotin]

R&D Systems | Catalog # BAF2827

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all ESAM Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ESAM
Gln30-Ser248
Accession # Q925F2

Specificity

Detects mouse ESAM in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human ESAM is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse ESAM Antibody [Biotin]

Detection of ESAM in bEnd.3 cells by Flow Cytometry

bEnd.3 cells were stained with Goat Anti-Mouse ESAM Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2827, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse ESAM Antibody [Biotin]

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse ESAM

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ESAM

ESAM is a 55 kDa type I transmembrane glycoprotein belonging to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members are CXADR, CLMP, JAM-A-C, CD2, A33, and BT-IgSF. The extracellular region of ESAM contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion. Mouse ESAM extracellular domain shares 69% amino acid sequence identity with the corresponding region of human ESAM. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes and can be found associated with cell-to-cell junctions.

Long Name

Endothelial Cell Adhesion Molecule

Alternate Names

2310008D05Rik, endothelial cell adhesion molecule, endothelial cell-selective adhesion molecule, HUEL (C4orf1)-interacting protein, LP4791 protein, W117m

Entrez Gene IDs

90952 (Human); 69524 (Mouse)

Gene Symbol

ESAM

UniProt

Q925F2

Additional ESAM Products

Product Documents for Mouse ESAM Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ESAM Antibody [Biotin]

For research use only

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