Mouse G-CSF R/CD114 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse G‑CSF R/CD114 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GM-CSF R alpha and recombinant mouse GM-CSF R alpha is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse G‑CSF R/CD114
    Accession # P40223
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of G‑CSF R/CD114 in Mouse Bone Marrow by Flow Cytometry. Mouse bone marrow was stained with Rat Anti-Mouse Gr‑1/Ly‑6G PE‑conjugated Monoclonal Antibody (Catalog # FAB1037P) and either (A) Sheep Anti-Mouse G‑CSF R/CD114 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6039A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: G-CSF R/CD114
Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic and granulocyte lineage (1). G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Cell activation by G‑CSF is mediated by granulocyte colony stimulating factor receptor alpha (G-CSF R; also CD114), a 95‑105 kDa type I transmembrane protein and member of the cytokine receptor superfamily, type I cytokine receptor family, and type 2 subfamily of receptor proteins. Mouse G‑CSF R is synthesized as an 837 amino acid (aa) precursor that contains a 25 aa signal sequence, a 601 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 187 aa cytoplasmic tail. The ECD contains one Ig-like C2-type domain, five fibronectin type-III domains, and 11 potential sites for N‑linked glycosylation. Within the ECD there is also a WSXWS motif (aa 319‑323) that is necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding (2). Also, within the cytoplasmic domain there is a Box 1 motif which is required for JAK interaction and/or activation (1). Mouse G‑CSF R shares 63% aa sequence identity with human G‑CSF R. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia (1). These mutations typically lead to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophilic precursors in the bone marrow and neutropenia in peripheral blood (3). Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases (2). Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3 and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (4).
  • References:
    1. Ward, A.C. (2007) Front. Biosci. 12:608.   
    2. Layton, J.E. and N.E. Hall (2006) Front. Biosci. 11:3181.
    3. Mitsui, T. et al. (2003) Blood 101:2990.
    4. Nicola, N.A. in Cytokine Reference, 2001, Oppenheim, J.J. and M. Feldmann, eds. Academic Press p.1935.
  • Long Name:
    Granulocyte Colony Stimulating Factor Receptor
  • Entrez Gene IDs:
    1441 (Human); 12986 (Mouse)
  • Alternate Names:
    CD114 antigen; CD114; colony stimulating factor 3 receptor (granulocyte); CSF3R; Csfgr; G-CSF receptor; GCSFR; G-CSFR; GCSFRG-CSF-R; granulocyte colony-stimulating factor receptor
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Sheep IgG APC-conjugated Antibody

Ctrl IC016A 1  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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