Mouse G-CSFR/CD114 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6039A

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of G‑CSF R/CD114 in Mouse Bone Marrow by Flow Cytometry.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Mouse G-CSFR/CD114 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse G‑CSF R/CD114 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GM-CSF R alpha and recombinant mouse GM-CSF R alpha is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse G‑CSF R/CD114
Cys26-Asp626
Accession # P40223
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of G‑CSF R/CD114 in Mouse Bone Marrow by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Gr-1/Ly-6G PE-conjugated Monoclonal Antibody (FAB1037P) and either (A) Sheep Anti-Mouse G-CSF R/CD114 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6039A) or (B) Normal Sheep IgG Allophycocyanin Control (IC016A). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: G-CSFR/CD114

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic and granulocyte lineage (1). G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Cell activation by G‑CSF is mediated by granulocyte colony stimulating factor receptor alpha (G-CSF R; also CD114), a 95‑105 kDa type I transmembrane protein and member of the cytokine receptor superfamily, type I cytokine receptor family, and type 2 subfamily of receptor proteins. Mouse G‑CSF R is synthesized as an 837 amino acid (aa) precursor that contains a 25 aa signal sequence, a 601 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 187 aa cytoplasmic tail. The ECD contains one Ig-like C2-type domain, five fibronectin type-III domains, and 11 potential sites for N‑linked glycosylation. Within the ECD there is also a WSXWS motif (aa 319‑323) that is necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding (2). Also, within the cytoplasmic domain there is a Box 1 motif which is required for JAK interaction and/or activation (1). Mouse G‑CSF R shares 63% aa sequence identity with human G‑CSF R. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia (1). These mutations typically lead to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophilic precursors in the bone marrow and neutropenia in peripheral blood (3). Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases (2). Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3 and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (4).

References
  1. Ward, A.C. (2007) Front. Biosci. 12:608.   
  2. Layton, J.E. and N.E. Hall (2006) Front. Biosci. 11:3181.
  3. Mitsui, T. et al. (2003) Blood 101:2990.
  4. Nicola, N.A. in Cytokine Reference, 2001, Oppenheim, J.J. and M. Feldmann, eds. Academic Press p.1935.
  5.  
Long Name
Granulocyte Colony Stimulating Factor Receptor
Entrez Gene IDs
1441 (Human); 12986 (Mouse)
Alternate Names
CD114 antigen; CD114; colony stimulating factor 3 receptor (granulocyte); CSF3R; Csfgr; G-CSF R; G-CSF receptor; GCSFR; G-CSFR; GCSFRG-CSF-R; granulocyte colony-stimulating factor receptor

Product Datasheets

You must select a language.

x

Citations for Mouse G-CSFR/CD114 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Efficient differentiation of human neutrophils with recapitulation of emergency granulopoiesis in human G-CSF knockin humanized mice
    Authors: R Ito, I Katano, IWH Kwok, LG Ng, M Ida-Tanaka, Y Ohno, Y Mu, H Morita, E Nishinaka, C Nishime, M Mochizuki, K Kawai, TH Chien, Z Yunqian, F Yiping, LH Hua, T Celhar, JK Yen Chan, T Takahashi, M Goto, T Ogura, R Takahashi, M Ito
    Cell Reports, 2022-12-20;41(12):111841.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Conditionally pathogenic genetic variants of a hematopoietic disease-suppressing enhancer
    Authors: AA Soukup, DR Matson, P Liu, KD Johnson, EH Bresnick
    Science Advances, 2021-12-10;7(50):eabk3521.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Bone marrow drives central nervous system regeneration after radiation injury
    Authors: J Dietrich, N Baryawno, N Nayyar, YK Valtis, B Yang, I Ly, A Besnard, N Severe, KU Gustafsson, OC Andronesi, TT Batchelor, A Sahay, DT Scadden
    J. Clin. Invest., 2017-12-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse G-CSFR/CD114 APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse G-CSFR/CD114 APC-conjugated Antibody and earn rewards!

Have you used Mouse G-CSFR/CD114 APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review