Mouse Galectin-3 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1197P
Detection of Galectin‑3 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (2)
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Mouse Galectin-3 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Galectin-3 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant human (rh) Galectin-3 and no cross-reactivity with rhGalectin-2, -4, -8 or recombinant mouse Galectin-1, -4, or -7.
Source
Monoclonal Rat IgG2A Clone # 202213
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Galectin‑3
Ala2-Ile264
Accession # P16110
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Galectin-3 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Galectin‑3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Integrin aM/CD11b APC-conjugated Monoclonal Antibody (Catalog # FAB1124A) and either (A) Rat Anti-Mouse Galectin-3 PE-conjugated Monoclonal Antibody (Catalog # FAB1197P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified as either prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; or tandem-repeat galectins (-4, -6, -8, -9, -12) that consist of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to act extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins. Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. In particular, it is reported to bind NG2 when complexed to Integrin alpha 3 beta 1 on endothelial  cells, initiating cell motility and angiogenesis. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces Th1 type and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity.

Entrez Gene IDs
3958 (Human); 16854 (Mouse)
Alternate Names
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3

Product Datasheets

Citations for Mouse Galectin-3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Association of Cardiac Galectin-3 Expression, Myocarditis, and Fibrosis in Chronic Chagas Disease Cardiomyopathy
    Authors: BS de Freitas, DN Silva, RH Carvalho, GL de Almeida, BD Paredes, L Aragão Fra, CM Azevedo, JF Vasconcelo, CS Meira, PC Neto, SG Macambira, KN da Silva, KJ Allahdadi, F Tavora, JD de Souza N, RR Dos Santos, MB Soares
    Am. J. Pathol, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells.
    Authors: Ansa-Addo EA, Lange S, Stratton D, Antwi-Baffour S, Cestari I, Ramirez MI, McCrossan MV, Inal JM
    J. Immunol., 2010;185(9):5236-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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