Mouse Integrin  alpha M/CD11b APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1124A-100
FAB1124A-025
Detection of Integrin  alpha M/CD11b in Mouse Bone Marrow Cells by Flow Cytometry.
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Mouse Integrin  alpha M/CD11b APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha M/CD11b. Cross-reaction with human Integrin alpha M has been reported (1, 2).
Source
Monoclonal Rat IgG2B Clone # M1/70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Con A-activated C57BL/10 splenocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Integrin  alpha M/CD11b in Mouse Bone Marrow Cells by Flow Cytometry. View Larger

Detection of Integrin  alpha M/CD11b in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Gr‑1/Ly‑6G PE‑conjugated Monoclonal Antibody (Catalog # FAB1037P) and either (A) Rat Anti-Mouse Integrin  alpha M/CD11b APC‑conjugated Monoclonal Antibody (Catalog # FAB1124A) or (B) Rat IgG2B Allophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha  and a beta  subunit. The Integrin  alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin  alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin  alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

References
  1. Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
  2. Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
Entrez Gene IDs
3684 (Human); 16409 (Mouse)
Alternate Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide); Cell surface glycoprotein MAC-1 subunit alpha; CR-3 alpha chain; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

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