Mouse Integrin alpha M/CD11b APC‑conjugated Antibody

Name: Mouse Integrin alpha M/CD11b APC‑conjugated Antibody
Brand: R&D Systems
SKU: FAB1124A
Price: 139 USD
Availability: InStock

R&D Systems | Catalog # FAB1124A

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG_2B Clone # M1/70

View all CD11b/Integrin alpha M Primary Antibodies »

Product Specifications

Immunogen

Con A-activated C57BL/10 splenocytes

Specificity

Detects mouse Integrin alpha M/CD11b. Cross-reaction with human Integrin alpha M has been reported (1, 2).

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse Integrin alpha M/CD11b APC‑conjugated Antibody

Detection of Integrin alpha M/CD11b in Mouse Bone Marrow Cells by Flow Cytometry.

Mouse bone marrow cells were stained with Rat Anti-Mouse Gr-1/Ly-6G PE-conjugated Monoclonal Antibody (Catalog # FAB1037P) and either (A) Rat Anti-Mouse Integrin aM/CD11b APC-conjugated Monoclonal Antibody (Catalog # FAB1124A) or (B) Rat IgG_2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Integrin alpha M/CD11b APC‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse bone marrow cells

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.