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Mouse GDF-1 Propeptide Alexa Fluor® 488-conjugated Antibody

Catalog #: AF3069G Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3069G-100UG

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Mouse GDF-1 Propeptide Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GDF-1 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant mouse (rm) GDF-1 and rmGDF-3 Propeptide is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse GDF-1 Propeptide
Pro25-Leu235
Accession # P20863
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GDF-1

Mouse GDF-1 Propeptide is a 26 kDa, secreted monomeric glycopeptide. It is synthesized as part of a 357 amino acid (aa) preprocursor that contains a 23 aa signal sequence, a 214 aa Propeptide and a 120 aa mature molecule. Following secretion, the proregion is presumably proteolytically cleaved from the mature molecule at an Arg-Arg dibasic residue motif. The amino acid sequence of mouse GDF-1 Propeptide is 65% and 78% identical to that of human and rat GDF-1 Propeptide, respectively.

Long Name
Growth Differentiation Factor 1
Entrez Gene IDs
2657 (Human); 14559 (Mouse)
Alternate Names
DORV; DTGA3; embryonic growth/differentiation factor 1; GDF1; GDF-1; growth differentiation factor 1

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