Mouse GDF-1 Propeptide Antibody

R&D Systems | Catalog # AF3069

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GDF-1 Propeptide
Pro25-Leu235
Accession # P20863

Specificity

Detects mouse GDF-1 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant mouse (rm) GDF-1 and rmGDF-3 Propeptide is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse GDF-1 Propeptide Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse GDF‑1 Propeptide

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-1

Mouse GDF-1 Propeptide is a 26 kDa, secreted monomeric glycopeptide. It is synthesized as part of a 357 amino acid (aa) preprocursor that contains a 23 aa signal sequence, a 214 aa Propeptide and a 120 aa mature molecule. Following secretion, the proregion is presumably proteolytically cleaved from the mature molecule at an Arg-Arg dibasic residue motif. The amino acid sequence of mouse GDF-1 Propeptide is 65% and 78% identical to that of human and rat GDF-1 Propeptide, respectively.

Long Name

Growth Differentiation Factor 1

Alternate Names

GDF1

Entrez Gene IDs

2657 (Human); 14559 (Mouse)

Gene Symbol

GDF1

UniProt

Additional GDF-1 Products

Product Documents for Mouse GDF-1 Propeptide Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse GDF-1 Propeptide Antibody

For research use only

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