Mouse GITR/TNFRSF18 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB52412N Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB52412N-100UG

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Mouse GITR/TNFRSF18 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GITR/TNFRSF18 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2375B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse GITR/TNFRSF18
Accession # O35714
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HEK293 Human Cell Line Transfected with Mouse GITR/TNFRSF18 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor; also named AITR) is a member of the co‑stimulatory subset of the TNF receptor superfamily (1, 2). In mouse, the GITR gene is composed of five exons and encodes multiple length isoforms that arise from alternate splicing. The “standard”, or first reported isoform is a type I transmembrane protein, 228 amino acids (aa) in length that contains a 19 aa signal sequence, a 134 aa extracellular region, a 21 aa transmembrane segment, and a 54 aa cytoplasmic domain. The extracellular region contains four potential N-linked glycosylation sites plus three cysteine-rich pseudorepeats of about 40 aa each (3, 4). The extracellular regions of mouse and human are 57% aa identical. The cytoplasmic domain has a P-x-Q/E-E motif that is known to associate with TRAF2. This is a common characteristic of TNFRSF members with co‑stimulatory functions (4). Three other mouse GITR isoforms (B, C and D) have been reported (5). All share the same N-terminal 101 of 134 aa in the extracellular region (including pseudorepeats #1, #2 and one-half of #3). Isoform D diverges at aa #101 and continues for another 12 aa for a total length of 113 aa. This is a naturally-occurring soluble form. Isoforms B and C show splicing in their cytoplasmic tails that creates cytoplasmic domains of 118 aa and 46 aa, respectively. In both the B and C isoforms, the TRAF2 binding site is spliced out, with a p56lck binding site inserted in isoform B (4). Given its membership in the TNFRSF, it likely functions as a trimer on the cell surface (2). GITR is predominantly expressed on CD4+CD25+ regulatory T cells (Treg) and naïve CD8+ and CD4+ CD25- T cells, where its expression is up-regulated after antigen-driven activation. GITR activation provides co‑stimulatory signals for activated CD4+ CD25- T cells to enhance cell proliferation and augment cytokine production (IL-2, IL-4, IFN-gamma ). On CD4+ CD25+ Treg cells, GITR activation provides co‑stimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state (6‑8).

References
  1. Kwon, B. et al. (2003) Exp. Mol. Med. 35:8. 
  2. Croft, M. (2003) Nat. Rev. Immunol. 3:609. 
  3. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
  4. Nocentini, G. et al. (2000) DNA Cell Biol. 19:205. 
  5. Nocentini, G. et al. (2000) Cell Death Differ. 7:408.
  6. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
  7. Ji, H. et al. (2004) J. Immunol. 172:5823.
  8. Stephens, G.L. et al. (2004) 173:5008.
Long Name
Glucocorticoid Induced TNF Receptor Family Related Gene
Entrez Gene IDs
8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
Alternate Names
Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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