Mouse ICAM-1/CD54 Quantikine ELISA Kit

  (24 citations)     
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Assay Procedure
Citations (24)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Homogenates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.057 ng/mL
  • Assay Range
    0.3 - 20 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse sICAM-1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse sICAM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sICAM-1 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed, recombinant mouse sICAM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sICAM-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.99 2.61 8.34 1.23 2.84 7.87
Standard Deviation 0.1 0.15 0.76 0.09 0.16 0.6
CV% 10.1 5.7 9.1 7.3 5.6 7.6

Recovery

The recovery of mouse sICAM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 104 98-113
EDTA Plasma (n=7) 98 83-113
Heparin Plasma (n=7) 104 85-120
Serum (n=6) 95 80-119
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse sICAM-1 in each matrix were diluted with Calibrator Diluent and then assayed.
 ICAM-1/CD54 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ICAM-1/CD54
ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.
  • Long Name:
    Intercellular Adhesion Molecule 1
  • Entrez Gene IDs:
    3383 (Human); 15894 (Mouse); 25464 (Rat)
  • Alternate Names:
    BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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Species
Sample Type
  1. Alleviation of secondary brain injury, posttraumatic inflammation, and brain edema formation by inhibition of factor XIIa
    Authors: S Hopp, MW Nolte, C Stetter, C Kleinschni, AL Sirén, C Albert-Wei
    J Neuroinflammation, 2017;14(1):39.
    Species: Mouse
    Sample Type: Plasma
  2. Simvastatin attenuates endothelial activation through 15-epi-lipoxin A4 production in murine chronic Chagas cardiomyopathy
    Authors: Fabiola González-H
    Antimicrob. Agents Chemother, 2016;0(0):.
    Species: Mouse
    Sample Type: Serum
  3. Endothelial mineralocorticoid receptor activation enhances endothelial protein C receptor and decreases vascular thrombosis in mice.
    Authors: Lagrange J, Li Z, Fassot C, Bourhim M, Louis H, Nguyen Dinh Cat A, Parlakian A, Wahl D, Lacolley P, Jaisser F, Regnault V
    FASEB J, 2014;28(5):2062-72.
    Species: Mouse
    Sample Type: Plasma
  4. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012;7(0):87-104.
    Species: Mouse
    Sample Type: BALF
  5. Abcg2 deficiency augments oxidative stress and cognitive deficits in Tg-SwDI transgenic mice.
    J. Neurochem., 2012;233(0):456-469.
    Species: Mouse
    Sample Type: Serum
  6. Overexpression of sICAM-1 in the Alveolar Epithelial Space Results in an Exaggerated Inflammatory Response and Early Death in Gram Negative Pneumonia.
    Authors: Mendez MP, Monroy YK, Du M
    Respir. Res., 2011;12(1):12.
    Species: Mouse
    Sample Type: BALF
  7. Early protection from burn-induced acute lung injury by deletion of preprotachykinin-A gene.
    Authors: Sio SW, Moochhala S, Lu J
    Am. J. Respir. Crit. Care Med., 2010;181(1):36-46.
    Species: Mouse
    Sample Type: Tissue Homogenates
  8. Early atherosclerosis and vascular inflammation in mice with diet-induced type 2 diabetes.
    Authors: Bartels ED, Bang CA, Nielsen LB
    Eur. J. Clin. Invest., 2009;39(3):190-9.
    Species: Mouse
    Sample Type: Plasma
  9. Role of the heparan sulfate proteoglycan syndecan-1 (CD138) in delayed-type hypersensitivity.
    Authors: Kharabi Masouleh B, ten Dam GB, Wild MK, Seelige R, van der Vlag J, Rops AL, Echtermeyer FG, Vestweber D, van Kuppevelt TH, Kiesel L, Gotte M
    J. Immunol., 2009;182(8):4985-93.
    Species: Mouse
    Sample Type: Tissue Homogenates
  10. Prevention of ocular inflammation in endotoxin-induced uveitis with resveratrol by inhibiting oxidative damage and nuclear factor-kappaB activation.
    Authors: Kubota S, Kurihara T, Mochimaru H, Satofuka S, Noda K, Ozawa Y, Oike Y, Ishida S, Tsubota K
    Invest. Ophthalmol. Vis. Sci., 2009;50(7):3512-9.
    Species: Mouse
    Sample Type: Cell Lysates
  11. (Pro)renin receptor promotes choroidal neovascularization by activating its signal transduction and tissue renin-angiotensin system.
    Authors: Satofuka S, Ichihara A, Nagai N, Noda K, Ozawa Y, Fukamizu A, Tsubota K, Itoh H, Oike Y, Ishida S
    Am. J. Pathol., 2008;173(6):1911-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
  12. A neutralizing antibody against receptor for advanced glycation end products (RAGE) reduces atherosclerosis in uremic mice.
    Authors: Bro S, Flyvbjerg A, Binder CJ, Bang CA, Denner L, Olgaard K, Nielsen LB
    Atherosclerosis, 2008;201(2):274-80.
    Species: Mouse
    Sample Type: Plasma
  13. Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin.
    Authors: Izumi-Nagai K, Nagai N, Ohgami K, Satofuka S, Ozawa Y, Tsubota K, Ohno S, Oike Y, Ishida S
    Invest. Ophthalmol. Vis. Sci., 2008;49(4):1679-85.
    Species: Mouse
    Sample Type: Tissue Homogenates
  14. Aspergillus fumigatus stimulates leukocyte adhesion molecules and cytokine production by endothelial cells in vitro and during invasive pulmonary disease.
    Authors: Chiang LY, Sheppard DC, Gravelat FN, Patterson TF, Filler SG
    Infect. Immun., 2008;76(8):3429-38.
    Species: Mouse
    Sample Type: Tissue Homogenates
  15. Endogenous hydrogen sulfide regulates leukocyte trafficking in cecal ligation and puncture-induced sepsis.
    Authors: Zhang H, Zhi L, Moochhala SM, Moore PK, Bhatia M
    J. Leukoc. Biol., 2007;82(4):894-905.
    Species: Mouse
    Sample Type: Tissue Homogenates
  16. Important role of apoptosis signal-regulating kinase 1 in ischemic acute kidney injury.
    Authors: Terada Y, Inoshita S, Kuwana H, Kobayashi T, Okado T, Ichijo H, Sasaki S
    Biochem. Biophys. Res. Commun., 2007;364(4):1043-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
  17. Macular pigment lutein is antiinflammatory in preventing choroidal neovascularization.
    Authors: Izumi-Nagai K, Nagai N, Ohgami K, Satofuka S, Ozawa Y, Tsubota K, Umezawa K, Ohno S, Oike Y, Ishida S
    Arterioscler. Thromb. Vasc. Biol., 2007;27(12):2555-62.
    Species: Mouse
    Sample Type: Tissue Homogenates
  18. Cell-based angiopoietin-1 gene therapy for acute lung injury.
    Authors: McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ
    Am. J. Respir. Crit. Care Med., 2007;175(10):1014-26.
    Species: Mouse
    Sample Type: Plasma
  19. Role of peroxisome proliferator-activated receptor-alpha in acute pancreatitis induced by cerulein.
    Authors: Genovese T, Mazzon E, Di Paola R, Muia C, Crisafulli C, Malleo G, Esposito E, Cuzzocrea S
    Immunology, 2006;118(4):559-70.
    Species: Mouse
    Sample Type: Tissue Homogenates
  20. Dietary arginine enhances adhesion molecule and T helper 2 cytokine expression in mice with gut-derived sepsis.
    Authors: Yeh CL, Hsu CS, Chiu WC, Hou YC, Yeh SL
    Shock, 2006;25(2):155-60.
    Species: Mouse
    Sample Type: Plasma
  21. Combination of systemic thioacetamide (TAA) injections and ethanol feeding accelerates hepatic fibrosis in C3H/He mice and is associated with intrahepatic up regulation of MMP-2, VEGF and ICAM-1.
    Authors: Kornek M, Raskopf E, Guetgemann I, Ocker M, Gerceker S, Gonzalez-Carmona MA, Rabe C, Sauerbruch T, Schmitz V
    J. Hepatol., 2006;45(3):370-6.
    Species: Mouse
    Sample Type: Tissue Homogenates
  22. Selective suppression of pathologic, but not physiologic, retinal neovascularization by blocking the angiotensin II type 1 receptor.
    Authors: Nagai N, Noda K, Urano T, Kubota Y, Shinoda H, Koto T, Shinoda K, Inoue M, Shiomi T, Ikeda E, Tsubota K, Suda T, Oike Y, Ishida S
    Invest. Ophthalmol. Vis. Sci., 2005;46(3):1078-84.
    Species: Mouse
    Sample Type: Tissue Homogenates
  23. Suppression of graft coronary artery disease by a brief treatment with a selective epsilonPKC activator and a deltaPKC inhibitor in murine cardiac allografts.
    Authors: Tanaka M, Terry RD, Mokhtari GK, Inagaki K, Koyanagi T, Kofidis T, Mochly-Rosen D, Robbins RC
    Circulation, 2004;110(11):II194-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
  24. Cardiomyocyte-specific Bcl-2 overexpression attenuates ischemia-reperfusion injury, immune response during acute rejection, and graft coronary artery disease.
    Authors: Tanaka M, Nakae S, Terry RD, Mokhtari GK, Gunawan F, Balsam LB, Kaneda H, Kofidis T, Tsao PS, Robbins RC
    Blood, 2004;104(12):3789-96.
    Species: Mouse
    Sample Type: Tissue Homogenates
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