Detection of ICOS in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes treated with 1 μg/mL Concanavalin A for 3 days were stained with Rat Anti-Mouse ICOS APC‑conjugated Monoclonal Antibody (Catalog # FAB168A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of ICOS in Activated Mouse Splenocytes by Flow Cytometry.
Activated mouse splenocytes were stained with Rat Anti-Mouse ICOS APC-conjugated Monoclonal Antibody (Catalog # FAB168A) and either (A) Rat Anti-Mouse 4-1BB/TNFRSF9/CD137 PE-conjugated Monoclonal Antibody (Catalog # FAB937P) or (B) Rat IgG2A Phycoerythrin Isotype Control(Catalog # IC006P).View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Mouse ICOS (inducible co-stimulator), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Mouse ICOS is a homodimeric type I transmembrane protein consisting of 200 amino acids (aa) with a putative 20 aa signal sequence, a 122 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% aa similarity with CD 28 and CTLA-4. Mouse and human ICOS share approximately 72% aa identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is upregulated within approximately 24‑48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.