Mouse IFN-alpha 4 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB10259S Datasheet / COA / SDS

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FAB10259S-100UG

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All IFN-alpha 4 Antibodies

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Mouse IFN-alpha 4 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IFN-alpha 4 in direct ELISAs. In direct ELISAS, no cross-reactivity with recombinant mouse IFNA-1, -2, -6, -7, -9, -11, -12, -13, -15 or -16 is observed.

Source

Monoclonal Rat IgG2a Clone # 1020125

Purification

Protein A or G purified

Immunogen

Human embryonic kidney cell HEK293-derived mouse IFN-alpha 4
Leu23-Glu186
Accession # P07351

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IFN-alpha 4

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors (1). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them (4, 5). The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle (6). mIFN-A4 is a strong activator of Mx gene and was shown to be very effective in reducing splenomegaly (7).

Long Name

Interferon alpha 4

Entrez Gene IDs

3441 (Human); 15967 (Mouse)

Alternate Names

IFNA4; IFNalpha 4; IFN-alpha 4; IFN-alpha-4; IFN-alpha4a; INFA4; interferon alpha-4; Interferon alpha-4B; Interferon alpha-76; Interferon alpha-M1; interferon, alpha 4; MGC142200