Human TRA-1-81 Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TRA-1-81 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # MAB8495, filled histogram) or mouse IgM isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117).
TRA-1-81 in BG01V Human Embryonic Stem Cells. TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # MAB8495) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The TRA-1-81 antibody reacts with a high-molecular-mass carbohydrate epitope on the surface
of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem
(ES), and induced pluripotent stem (iPS) cells. TRA-1-81 is an epitope expressed on podocalyxin,
also known as Podocalyxin-Like Protein-1 (PCLP1 or PODXL), a type I
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Cell and Tissue Staining Kits
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