Human TRA-1-81 Antibody

Catalog # Availability Size / Price Qty
MAB8495
MAB8495-SP
Detection of TRA-1-81 in BG01V Human Cells by Flow Cytometry.
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Human TRA-1-81 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRA-1-81 in flow cytometry.
Source
Monoclonal Mouse IgM Clone # TRA-1-81
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Human embryonal carcinoma cell line 2102Ep
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of TRA-1-81 in BG01V Human Cells by Flow Cytometry. View Larger

Detection of TRA-1-81 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # MAB8495, filled histogram) or mouse IgM isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117).

Immunocytochemistry TRA-1-81 in BG01V Human Embryonic Stem Cells. View Larger

TRA-1-81 in BG01V Human Embryonic Stem Cells. TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # MAB8495) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRA-1-81

The TRA-1-81 antibody reacts with a high-molecular-mass carbohydrate epitope on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells.  TRA-1-81 is an epitope expressed on podocalyxin, also known as Podocalyxin-Like Protein-1 (PCLP1 or PODXL), a type I transmembrane glycoprotein.

Long Name
Tumor-related Antigen-1-81
Entrez Gene IDs
5420 (Human)
Alternate Names
gp200; MGC138240; PC; PCLP; PODXL; TRA181; TRA-1-81

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Reconstitution Buffers

Secondary Antibodies

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