Mouse IGSF9 Antibody

Catalog # Availability Size / Price Qty
MAB9140-100
MAB9140-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of IGSF9 in HEK293 Human Cell Line Transfected with Mouse IGSF9 and eGFP by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse IGSF9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGSF9 in direct ELISAs.
Source
Monoclonal Rat IgG2B Clone # 993107
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGSF9
Arg21-Leu730
Accession # Q05BQ1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IGSF9 antibody in HEK293 Human Cell Line Transfected with Mouse IGSF9 and eGFP antibody by Flow Cytometry. View Larger

Detection of IGSF9 in HEK293 Human Cell Line Transfected with Mouse IGSF9 and eGFP by Flow Cytometry. HEK293 human cell line transfected with (A) Mouse IGSF9 or (B) irrelevant protein, and eGFP was stained with Rat Anti-Mouse IGSF9 Monoclonal Antibody (Catalog # MAB9140) followed by APC-conjugated Goat anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on Rat IgG2B Isotype Control Antibody (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF9

IGSF9, also known as Dasm1, is an approximately 130 kDa transmembrane protein that plays a role in neuronal synapse maintenance and function (1). Mature mouse Dasm1 consists of a 714 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 424 aa cytoplasmic domain (2). Within the ECD, mouse Dasm1 shares 90% and 96% aa sequence identity with human and rat Dasm1, respectively. Alternative splicing generates an additional isoform that is truncated following the fifth Ig-like domain. Dasm1 is expressed in the dorsal root and trigeminal ganglia, forebrain, cortex, dentate gyrus, pyramidal cells, Purkinje cells, and hippocampal CA1 interneurons (2-5). It localizes to dendrites, cell bodies, and post-synaptic densities (3, 6). Dasm1 functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (5, 6).

References
  1. Hansen, M. and P.S. Walmod (2013) Neurochem. Res. 38:1236.
  2. Doudney, K. et al. (2002) Genomics 79:663.
  3. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
  4. Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
  5. Mishra, A. et al. (2014) J. Neurosci. 34:4187.
  6. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.
Long Name
Immunoglobulin Superfamily, Member 9
Entrez Gene IDs
57549 (Human); 93842 (Mouse); 304982 (Rat)
Alternate Names
Dasm1; FP18798; IGSF9; IGSF9A; immunoglobulin superfamily member 9A; immunoglobulin superfamily, member 9; KIAA1355; Nrt1; Protein Turtle Homolog A; TUTLA

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse IGSF9 Antibody

There are currently no reviews for this product. Be the first to review Mouse IGSF9 Antibody and earn rewards!

Have you used Mouse IGSF9 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review