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Mouse IL-13 R alpha 2 Quantikine ELISA Kit

    
Datasheet / CoA / SDS
Mouse IL-13 R alpha 2 Standard Curve

Mouse IL-13 R alpha 2 Standard Curve

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Mouse IL-13 R alpha 2 ELISA Linearity

Mouse IL-13 R alpha 2 ELISA Linearity

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Product Details
Assay Procedure
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Homogenates (13 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    5.6 pg/mL
  • Assay Range
    39.0 - 2,500 pg/mL
  • Specificity
    Natural and recombinant mouse IL-13 R alpha 2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse IL-13 R alpha 2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-13 R alpha 2 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse IL-13 R alpha 2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-13 R alpha 2 accurately. Results obtained using natural mouse IL-13 R alpha 2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-13 R alpha 2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 97.7 338 1011 101 279 1008
Standard Deviation 4.25 12.5 23.2 8.2 17.6 50.3
CV% 4.4 3.7 2.3 8.1 6.3 5

Recovery

The recovery of mouse IL-13 R alpha 2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 91 83-98
EDTA Plasma (n=4) 99 97-101
Heparin Plasma (n=4) 103 87-117
Serum (n=4) 97 83-108
Tissue Homogenates (n=4) 106 93-119
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-13 R alpha 2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
IL-13 R alpha 2 [HRP]
Product Datasheets
Product Datasheet

COA

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SDS
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-13 R alpha 2
Two members of the type 5 subfamily of type I cytokine receptors can serve as receptors for IL-13. IL-13 can bind to IL-13 R alpha 1 (CD213a1; previously designated IL-13 R alpha or NR4) with low affinity, then recruits the IL-4 R alpha chain to form a high affinity receptor, causing downstream STAT6 activation. Alternately, IL-13 can bind IL-13 R alpha 2 (CD213a2) with high affinity; this interaction does not cause activation of STAT6, but does induce TGF-beta production. IL-13 R alpha 1 and IL-13 R alpha 2 each have three extracellular fibronectin type III domains, two cytokine receptor homology modules and a WSXWS motif typical of the class I cytokine receptor family, but IL-13 R alpha 2 has a much shorter cytoplasmic tail. IL-13 R subunits can be expressed on monocytes, macrophages, fibroblasts, human B cells, basophils, eosinophils, endothelial cells, and smooth muscle cells.
  • Long Name:
    Interleukin 13 Receptor alpha 2
  • Entrez Gene IDs:
    3598 (Human); 16165 (Mouse)
  • Alternate Names:
    cancer/testis antigen 19; CD213a2 antigen; CD213a2; CT19; IL-13 R alpha 2; IL-13 receptor subunit alpha-2; IL13BP; IL13R alpha 2; IL-13R subunit alpha-2; IL13R; IL-13R; IL13RA2; IL-13Ra2; IL-13R-alpha-2; interleukin 13 binding protein; interleukin 13 receptor alpha 2 chain; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; Interleukin-13-binding protein
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, Control, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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