< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference observed with 1 or more available related molecules.
The Quantikine Mouse IL-13 R alpha 2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-13 R alpha 2 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse IL-13 R alpha 2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-13 R alpha 2 accurately. Results obtained using natural mouse IL-13 R alpha 2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-13 R alpha 2.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse IL-13 R alpha 2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Samples (n=4)
EDTA Plasma (n=4)
Heparin Plasma (n=4)
Tissue Homogenates (n=4)
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-13 R alpha 2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-13 R alpha 2
Two members of the type 5 subfamily of type I cytokine receptors can serve as receptors for IL-13. IL-13 can bind to IL-13 R alpha 1 (CD213a1; previously designated IL-13 R alpha or NR4) with low affinity, then recruits the IL-4 R alpha chain to form a high affinity receptor, causing downstream STAT6 activation. Alternately, IL-13 can bind IL-13 R alpha 2 (CD213a2) with high affinity; this interaction does not cause activation of STAT6, but does induce TGF-beta production. IL-13 R alpha 1 and IL-13 R alpha 2 each have three extracellular fibronectin type III domains, two cytokine receptor homology modules and a WSXWS motif typical of the class I cytokine receptor family, but IL-13 R alpha 2 has a much shorter cytoplasmic tail. IL-13 R subunits can be expressed on monocytes, macrophages, fibroblasts, human B cells, basophils, eosinophils, endothelial cells, and smooth muscle cells.
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